Metastasis Research Protocols

Volume I: Analysis of Cells and Tissues

  • Susan A. Brooks
  • Udo Schumacher

Part of the Methods in Molecular Medicine book series (MIMM, volume 57)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Detection Methods for Cellular Markers of Metastatic Potential

    1. Front Matter
      Pages 1-1
    2. Derek E. Roskell, Ian D. Buley
      Pages 3-11
    3. Amanda J. Atherton, Catherine Clarke
      Pages 41-48
    4. Lawrence John, Massimo Pignatelli
      Pages 75-98
    5. Girolamo Ranieri, Giampietro Gasparini
      Pages 99-113
    6. Helen E., Adrian L.
      Pages 115-121
    7. Richard S. Camplejohn
      Pages 133-143
    8. Christine Blancher, Adam Jones
      Pages 145-162
    9. Marta Toth, Rafael Fridman
      Pages 163-174
  3. Genetic Aspects of Metastasis

    1. Front Matter
      Pages 175-175
    2. Richard Poulsom
      Pages 177-197
    3. Hakan Goker, Janet Shipley
      Pages 199-221
    4. Rebecca Roylance
      Pages 223-240
    5. Adam Jones, Chisato Fujiyama, Kenneth Smith
      Pages 241-253
    6. Thelma Tennant, Marina Chekmareva, Carrie W. Rinker-Schaeffer
      Pages 255-270
    7. Richard Lilischkis, Hermann Kneitz, Hans Kreipe
      Pages 271-283
    8. Katja Haack, Krishna Soondrum, Michael Neumaier
      Pages 285-295
    9. Ruey HoKao, Giulio Francia
      Pages 297-305
  4. Mathematical Modeling of Metastasis

    1. Front Matter
      Pages 307-307
    2. Jonathan A. Sherratt
      Pages 309-315
  5. Back Matter
    Pages 317-322

About this book


Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe the key methods needed to investigate why and how metastasis occurs. Volume I of this two-volume set, Analysis of Cells and Tissues, presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. The work focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, and differential display. The second volume of this set, Analysis of Cell Behavior In Vitro and In Vivo, moves to the level of living cells and tissues to present methodologies applicable to examining metastatic behavior in vitro and in the whole animal.
Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.


Grading angiogenesis cells migration tissue

Editors and affiliations

  • Susan A. Brooks
    • 1
  • Udo Schumacher
    • 2
  1. 1.Research School of Biological and Molecular SciencesOxford Brookes UniversityOxfordUK
  2. 2.Institute for Anatomy, Department of NeuroanatomyUniversity Hospital Hamburg-EppendorfHamburgGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-610-9
  • Online ISBN 978-1-59259-136-7
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site