Ion Channel Localization

Methods and Protocols

  • Anatoli N. Lopatin
  • Colin G. Nichols

Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Pharmacological Labeling of Ion Channels and Receptors

    1. Front Matter
      Pages 17-17
    2. Cyrille Sur, John Atack
      Pages 39-56
    3. Xuenong Bo, Geoffrey Burnstock
      Pages 57-73
    4. Geula M. Bernstein, Owen T. Jones
      Pages 93-117
    5. Lourival D. Possani, Baltazar Becerril, Jan Tytgat, Muriel Delepierre
      Pages 145-165
  3. Applications of Green Fluorescent Protein (GFP) Technology

    1. Front Matter
      Pages 166-166
    2. Jan Eggermont, Dominique Trouet, Gunnar Buyse, Rudi Vennekens, Guy Droogmans, Bernd Nilius
      Pages 167-186
    3. Bryan D. Moyer, Bruce A. Stanton
      Pages 187-214
    4. Tooraj Mirshahi, Diomedes E. Logothetis, Massimo Sassaroli
      Pages 215-231
    5. Scott A. John, James N. Weiss
      Pages 233-248
    6. Elena N. Makhina, Colin G. Nichols
      Pages 261-274
    7. David C. Johns, Uta C. Hoppe, Eduardo Marbán, Brian O’Rourke
      Pages 275-296
  4. Functional Assays in Ion Channel Localization

    1. Front Matter
      Pages 296-296
    2. Cristóvão Albuquerque, Holly S. Engelman, C. Justin Lee, Amy B. MacDermott
      Pages 297-309
    3. Anatoli N. Lopatin, Elena N. Makhina, Colin G. Nichols
      Pages 311-325
    4. Fritjof Helmchen
      Pages 327-347
    5. Diana L. Pettit, George J. Augustine
      Pages 349-370
  5. Atomic Force Microscopy

    1. Front Matter
      Pages 370-370
    2. Hoeon Kim, Hai Lin, Ratneshwar Lal
      Pages 371-389
    3. Hans Oberleithner, Hermann Schillers, Stefan W. Schneider, Robert M. Henderson
      Pages 405-424
    4. Kenneth Snyder, Ping C. Zhang, Frederick Sachs
      Pages 425-459
    5. Daniel M. Czajkowsky, Zhifeng Shao
      Pages 461-481
  6. Back Matter
    Pages 483-502

About this book


Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies.
Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.

Editors and affiliations

  • Anatoli N. Lopatin
    • 1
  • Colin G. Nichols
    • 2
  1. 1.University of MichiganAnn Arbor
  2. 2.Washington University Medical SchoolSt. Louis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-833-2
  • Online ISBN 978-1-59259-118-3
  • Series Print ISSN 1557-2153
  • Series Online ISSN 1940-6053
  • Buy this book on publisher's site