Immunotoxin Methods and Protocols

  • Walter A. Hall

Part of the Methods in Molecular Biology™ book series (MIMB, volume 166)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Immunotoxin Construction and Development

    1. Front Matter
      Pages 1-1
    2. Ingegerd Hellström, Karl Erik Hellström, Peter D. Senter
      Pages 3-16
    3. Christopher A. Pennell, Mary E. Pauza
      Pages 17-29
    4. Luigi Barbieri, Andrea Bolognesi, Fiorenzo Stirpe
      Pages 71-85
    5. Ki-Uk Kim, Hsiao-Tzu Ni, Stephen R. Spellman, Kwan H. Cho, Walter C. Low, Walter A. Hall
      Pages 101-110
    6. Alan L. Lindstrom, Christopher A. Pennell
      Pages 125-135
  3. Oncologic Applications of Immunotoxins

    1. Front Matter
      Pages 136-136
    2. Walter A. Hall
      Pages 139-154
    3. Elise A. Sudbeck, Sutapa Ghosh, Xing-Ping Liu, Yaguo Zheng, Dorothea E. Myers, Fatih M. Uckun
      Pages 193-218
    4. S. Ramakrishnan, Robert Wild, Dana Nojima
      Pages 219-234
    5. Daniel A. Vallera
      Pages 235-246
  4. Alternative Applications of Immunotoxins

    1. Front Matter
      Pages 247-247
    2. Seth H. Pincus, Tamera K. Marcotte, Brent M. Forsyth, Hua Fang
      Pages 277-294
  5. Back Matter
    Pages 295-302

About this book


More potent and selective for killing cancer cells than chemotherapy, immunotoxins constitute a new class of human therapeutics that have widespread applications and a potential that has not yet been fully recognized. In Immunotoxin Methods and Protocols, leading experts from laboratories around the world describe in step-by-step detail their cutting-edge methods for developing and applying these powerful proteins. The conjugates created by these researchers contain entire monoclonal antibodies, single chain antibody fragments, and cytokines as their carrier ligands. Their toxic portion can include such conventional chemotherapeutics as doxirubicin, such ribosome-inactivating proteins as the ricin A chain or saporin, or such bacterial toxins as diptheria toxin. Also described are a variety of applications that demonstrate the versatility of these agents, including the treatment of primary malignant brain tumors, the generation of selective brain lesions to localize critical functions, and the targeting of the AIDS virus. Each readily reproducible method is optimized for experimental success with hands-on instructions, invaluable notes on pitfalls to avoid, and informative explanations of why certain steps are necessary.
Authoritative and highly practical, Immunotoxin Methods and Protocols provides today's cancer researchers, neuroscientists, and oncologists with state-of-the-art tools for exploring these powerful biological agents and their rapidly increasing applications.

Editors and affiliations

  • Walter A. Hall
    • 1
  1. 1.Department of NeurosurgeryUniversity of MinnesotaMinneapolis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-775-5
  • Online ISBN 978-1-59259-114-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site