Aging Methods and Protocols

  • Yvonne A. Barnett
  • Christopher R. Barnett

Part of the Methods in Molecular Medicine book series (MIMM, volume 38)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. The Study of Cell Senescence and Cell Death

    1. Front Matter
      Pages 21-21
    2. Bernard L. Strehler
      Pages 1-19
    3. Vincent J. Cristofalo, Craig Volker, Robert G. Allen
      Pages 23-52
    4. Graham Pawelec
      Pages 53-61
    5. Hector F. Valenzuela, Rita B. Effros
      Pages 63-70
    6. Ruaidhri J. Carmody, Ana P. Costa-Pereira, Sharon L. McKenna, Tom G. Cotter
      Pages 71-83
    7. Christopher J. Kirk, Richard A. Miller
      Pages 85-96
    8. Andrea Engel, Mahdi Adibzadeh, Graham Pawelec
      Pages 97-115
  3. Age-Related Changes to Xenobiotic Metabolizing Enzyme Systems

    1. Front Matter
      Pages 117-117
    2. Christopher R. Barnett, Costas Ioannides
      Pages 119-130
  4. Age-Related Changes to Defense Systems Against Biomolecule Damage

    1. Front Matter
      Pages 131-131
    2. Iris F. F. Benzie, John J. Strain
      Pages 133-142
    3. Peter H. Clingen, Jillian E. Lowe, Michael H. L. Green
      Pages 143-157
  5. Age-Related Changes to Cellular Biomolecules

    1. Front Matter
      Pages 169-169
    2. Sharon G. Wood, Catherine M. Gedik, Nicholas J. Vaughan, Andrew R. Collins
      Pages 171-178
    3. Yvonne A. Barnett, Christopher R. Barnett
      Pages 179-187
    4. Sai-Mei Hou
      Pages 189-197
    5. Jesus R. Requena, David L. Price, Suzanne R. Thorpe, John W. Baynes
      Pages 209-217
  6. Mitochondrial Morphology, Function, and Mutation with Age

    1. Front Matter
      Pages 219-219
    2. Jaime Miquel, Carlo Bertoni-Freddari
      Pages 221-235
    3. Federico V. Pallardo, Juan Sastre, Jaime Miquel, José Viña
      Pages 237-244
    4. Robert W. Taylor, Theresa M. Wardell, Emma L. Blakely, Gillian M. Borthwick, Elizabeth J. Brierley, Douglass M. Turnbull
      Pages 245-264
    5. Robert W. Taylor, Richard M. Andrews, Patrick F. Chinnery, Douglass M. Turnbull
      Pages 265-277
  7. Immune System and Aging

    1. Front Matter
      Pages 279-279
    2. Ian Beckman
      Pages 281-290
    3. Beatrix Grubeck-Loebenstein, Maria Saurwein-Teissl, Nikolaus Romani
      Pages 291-309
    4. Erminia Mariani, Corona Alonso, Rafael Solana
      Pages 311-320
    5. Derek Middleton, Martin D. Curran, Fionnuala Williams
      Pages 321-349
  8. Case Studies

    1. Front Matter
      Pages 351-351
    2. Byung Pal Yu
      Pages 353-359
  9. Back Matter
    Pages 379-385

About this book


With rapidly rising life expectancies and a general lack of understanding about the aging process, the need to treat geriatric diseases is becoming an ever more significant private and public health issue. In Aging Methods and Protocols, Yvonne and Christopher Barnett and a team of recognized international experts detail key biochemical, analytical, and molecular techniques for the investigation of aging at the cellular, tissue, organ, and whole system levels. These cutting-edge methods address a wide range of research needs, from uncovering the factors associated with cell senescence and death, to exploring alterations in the body's ability both to metabolize xenobiotics, and to defend itself against biomolecular damage. State-of-the-art protocols also measure the morphological, functional, and molecular changes that accumulate within mitochrondria over time, and permit the genetic and functional characteristics of the immune system to be determined. Two important case studies examine the role of dietary restriction on life span extension and the use of transgenic animals in the molecular analysis of aging.
Wide ranging and highly practical, Aging Methods and Protocols provides today's molecular gerontologists, pharmacologists, and clinical investigators with a gold-standard collection of readily reproducible techniques for identifying those key cellular and molecular processes that might one day make it possible to regulate the aging process.

Editors and affiliations

  • Yvonne A. Barnett
    • 1
  • Christopher R. Barnett
    • 1
  1. 1.School of Biomedical SciencesUniversity of UlsterNorthern Ireland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-582-9
  • Online ISBN 978-1-59259-070-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site