Developmental Biology Protocols

  • Rocky S. Tuan
  • Cecilia W. Lo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 137)

Table of contents

  1. Front Matter
    Pages i-xxv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Rocky S. Tuan, Cecilia W. Lo
      Pages 3-5
  3. Manipulation Of Developmental Gene Expression and Function

    1. Front Matter
      Pages 7-7
    2. Elizabeth L. Wilder
      Pages 9-14
    3. Jenny E. Rooke, Nicole A. Theodosiou, Tian Xu
      Pages 15-22
    4. Peter G. Alexander, George L. Barnes, Rocky S. Tuan
      Pages 23-36
    5. Steven A. Oberlender, Rocky S. Tuan
      Pages 37-42
  4. Analysis of Gene Expression

    1. Front Matter
      Pages 43-43
    2. Troy A. Giambernardi, Robert J. Klebe
      Pages 51-58
    3. Asad U. Sheikh, Bruno M. Polliotti, Richard K. Miller
      Pages 75-86
    4. Stefan Wawersik, Jonathan A. Epstein
      Pages 87-96
    5. Antoon F. M. Moorman, Piet A. J. De Boer, Jan M. Ruijter, Jaco Hagoort, Diego Franco, Wouter H. Lamers
      Pages 97-115
    6. Giselbert Hauptmann, Thomas Gerster
      Pages 139-148
    7. Ronald A. Conlon
      Pages 149-152
    8. Joseph C. Ayoob, Jean M. Sanger, Joseph W. Sanger
      Pages 153-157

About this book

Introduction

The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.

Editors and affiliations

  • Rocky S. Tuan
    • 1
  • Cecilia W. Lo
    • 2
  1. 1.Thomas Jefferson UniversityPhiladelphia
  2. 2.University of PennsylvaniaPhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/1592590667
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-854-7
  • Online ISBN 978-1-59259-066-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book