© 2000

Mass Spectrometry of Proteins and Peptides

Mass Spectrometry of Proteins and Peptides

  • John R. Chapman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 146)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. John R. Yates III, Andrew J. Link, David Schieltz
    Pages 17-26
  3. Haydar Karaoglu, Ian Humphery-Smith
    Pages 63-94
  4. John R. Engen, David L. Smith
    Pages 95-112
  5. Keiryn L. Bennett, Thomas Matthiesen, Peter Roepstorff
    Pages 113-131
  6. Igor A. Kaltashov, Aiqun Li, Zoltán Szilágyi, Károly Vékey, Catherine Fenselau
    Pages 133-146
  7. Pasquale Ferranti, Gianfranco Mamone, Antonio Malorni
    Pages 147-165
  8. H. Peter Happersberger, Marcus Bantscheff, Stefanie Barbirz, Michael O. Glocker
    Pages 167-184
  9. Carol E. Parker, Kenneth B. Tomer
    Pages 185-201
  10. Richard W. Kriwacki, Gary Siuzdak
    Pages 223-238
  11. Andrew N. Krutchinsky, Ayeda Ayed, Lynda J. Donald, Werner Ens, Harry W. Duckworth, Kenneth G. Standing
    Pages 239-249
  12. Fred Rosche, Jörn Schmidt, Torsten Hoffmann, Robert P. Pauly, Christopher H.S. McIntosh, Raymond A. Pederson et al.
    Pages 251-272
  13. Ekaterina Mirgorodskaya, Thomas N. Krogh, Peter Roepstorff
    Pages 273-292
  14. Reto Stöcklin, Jean-François Arrighi, Khan Van Hoang-, Lan Vu, Fabrice Cerini, Nicolas Gilles et al.
    Pages 293-315
  15. Reto Stöcklin, Dietrich Mebs, Jean-Claude Boulain, Pierre-Alain Panchaud, Henri Virelizier, Cécile Gillard-Factor
    Pages 317-335

About this book


New types of mass spectrometric instrumentation and new ionization techniques have dramatically extended the applicability of mass spectrometry to the analysis of proteins and peptides. In Mass Spectrometry of Proteins and Peptides, John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in this area. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing.
Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable.

Editors and affiliations

  • John R. Chapman
    • 1
  1. 1.SaleManchesterUK

Bibliographic information

  • Book Title Mass Spectrometry of Proteins and Peptides
  • Book Subtitle Mass Spectrometry of Proteins and Peptides
  • Editors John R. Chapman
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-609-3
  • Softcover ISBN 978-1-4899-4154-1
  • eBook ISBN 978-1-59259-045-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 538
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site


"John Chapman has followed on from his excellent earlier volume 'Protein and Peptide Analysis by Mass Spectrometry' by bringing together in this both very readable and informative volume 27 individual chapters from acknowledged practitioners of the application of mass spectrometry to peptides and proteins. Each chapter is packed with real information both for the biologist and mass spectroscopist and contains a wealth of detail on sample reparation and the subsequent interpretation of the MS results. ...The 'hot' topics of the investigation of non-covalent complexes, protein-protein interactions and higher order protein structures by mass spectrometry are addressed in separate chapters. Any laboratory working on peptides and proteins whether they use MS or not should have access to this book. It is a goldmine."-European Peptide Society Newsletter

"...a well crafted book using the experience of respected pioneers of modern mass spectrometric methods....Many of the methods used in proteomic studies rely on mass spectrometric analysis of proteins and peptides, as well as protein separation technology. The latter is dealt with to some extent by this volume; however, the mass spectrometric aspects are very well covered....This volume shows the great versatility of mass spectrometric analysis, in this case related to protein and peptides, indicating that most analytical challenges can be met by the technique, especially when combined with innovative sample preparation. For the clinical biochemist with an interest in proteins and peptides, this volume is an important reference. For other biochemists it represents a view of a great potential, which will develop as our use of the human genome information progresses."-Annals of Clinical Biochemistry

"The availability of a compilation of authoritative reviews that include a wealth of practical detail regarding sample preparation and presentation as well as instrument operation is both timely and of considerable value. In total there are some twenty-seven comprehensive descriptions of mass spectrometric analyses of proteins, peptides and mixtures of various complexity and provenance. The elucidation of higher-order structure is described as well as primary sequence determination, epitope mapping and cell typing using several substrates from highly refined isolates to more complex mixtures and whole cells. The contributions are provided by over sixty well established researchers and include detailed information for sample isolation and preparation as well as a comprehensive bibliography on each topic area. Further information is collated into a series of appendices covering an abbreviation glossary, listings of mass/abundance values for elements, amino acids and monosaccharide residues and a literature update on protein mass spectrometry for 1997 - 1998. This volume is a valuable addition to the series: it is comprehensive in terms of the range of applications considered and extensive in the descriptions of the analytical process. It is unusual among reviews of mass spectrometric applications for the wealth of experimental detail provided an this aspect alone makes it an essential item for every biological mass spectrometry and proteomics laboratory." - Chromatographia