Natural Killer Cell Protocols

Cellular and Molecular Methods

  • Kerry S. Campbell
  • Marco Colonna

Part of the Methods in Molecular Biology book series (MIMB, volume 121)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Marina Cella, Marco Colonna
    Pages 1-4
  3. Werner Held, Bente Lowin-Kropf, David H. Raulet
    Pages 5-12
  4. Hergen Spits, Pieter Res, Ana-Cristina Jaleco
    Pages 25-38
  5. Zuhair K. Ballas, Wendy L. Rasmussen
    Pages 39-47
  6. Jingxuan Liu, Thaddeus George, Gene A. Devora, P. V. Sivakumar, Colleen Davenport, Wayne C. Lai et al.
    Pages 61-71
  7. P. V. Sivakumar, Thaddeus George, Igor J. Puzanov, Noelle S. Williams, Susan Stepp, Jingxuan Liu et al.
    Pages 73-79
  8. Per H. Basse, Theresa L. Whiteside, Ronald B. Herberman
    Pages 81-94
  9. William H. Chambers, Simon M. Watkins, Per H. Basse
    Pages 95-114
  10. Miriam E. Berman, William A. Muller
    Pages 115-123
  11. Alison Darmon, Lesley J. Page, Gillian M. Griffiths
    Pages 145-153
  12. Marek Los, Henning Walczak, Klaus Schulze-Osthoff, John C. Reed
    Pages 155-162
  13. Anthony A. Scalzo, Helen E. Farrell, Gunasegaran Karupiah
    Pages 163-177
  14. Matthew J. Loza, Jeffrey S. Faust, Bice Perussia
    Pages 193-209
  15. Wayne C. Lai, Ying Zhang, Thaddeus George, P. V. Sivakumar, Susan Stepp, Deming Zhou et al.
    Pages 211-217
  16. Barbara Uchanska-Ziegler, Qiao Xugang, Armin Volz, Ahuva Nissim, Andreas Ziegler
    Pages 219-237
  17. Christine C. Winter, Eric O. Long
    Pages 239-250
  18. Michael J. Wilson, Michaela Torkar, John Trowsdale
    Pages 251-263
  19. James C. Ryan, Eréne C. Niemi, Mary C. Nakamura
    Pages 283-295
  20. Bryce A. Binstadt, Brandi L. Williams, Robert T. Abraham, Paul J. Leibson
    Pages 297-304
  21. Frédéric Vély, Alain Trautmann, Eric Vivier
    Pages 313-322
  22. Toru Miyazaki, Kerry S. Campbell
    Pages 323-335
  23. Sandra Ristow, Donald L. Evans, Liliana Jaso-Friedmann
    Pages 347-357
  24. Albert E. Munson, Kathleen E. Phillips
    Pages 359-365
  25. Back Matter
    Pages 368-398

About this book


In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

Editors and affiliations

  • Kerry S. Campbell
    • 1
  • Marco Colonna
    • 2
  1. 1.Fox Chase Cancer CenterPhiladelphia
  2. 2.Basel Institute for ImmunologyBaselSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-683-3
  • Online ISBN 978-1-59259-044-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site