Aqueous Two-Phase Systems: Methods and Protocols

Methods and Protocols

  • Rajni Hatti-Kaul

Part of the Methods in Biotechnology™ book series (MIBT, volume 11)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Preparation and Characterization of Aqueous Two-Phase Systems

    1. Rajni Hatti-Kaul
      Pages 1-10
    2. Anita Kaul
      Pages 11-21
    3. Daniel Forciniti
      Pages 23-33
    4. Donald E. Brooks, Raymond Norris Jones
      Pages 35-45
  3. Partitioning Techniques

    1. Front Matter
      Pages 11-45
    2. Telma Teixeira Franco, Rajni Hatti-Kaul
      Pages 47-54
    3. Hans-Erik Åkerlund
      Pages 55-64
    4. Ulla-Britt Hansson, Christer Wingren
      Pages 65-75
  4. Partitioning of Soluble Molecules and Particulates

About this book

Introduction

In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented.
Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Editors and affiliations

  • Rajni Hatti-Kaul
    • 1
  1. 1.Center for Chemistry and Chemical EngineeringLund UniversityLundSweden

Bibliographic information

  • DOI https://doi.org/10.1385/1592590284
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-541-6
  • Online ISBN 978-1-59259-028-5
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • About this book