Aqueous Two-Phase Systems: Methods and Protocols

Methods and Protocols

  • Rajni Hatti-Kaul

Part of the Methods in Biotechnology™ book series (MIBT, volume 11)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Preparation and Characterization of Aqueous Two-Phase Systems

    1. Rajni Hatti-Kaul
      Pages 1-10
    2. Anita Kaul
      Pages 11-21
    3. Daniel Forciniti
      Pages 23-33
    4. Donald E. Brooks, Raymond Norris Jones
      Pages 35-45
  3. Partitioning Techniques

    1. Front Matter
      Pages 11-45
    2. Telma Teixeira Franco, Rajni Hatti-Kaul
      Pages 47-54
    3. Hans-Erik Åkerlund
      Pages 55-64
    4. Ulla-Britt Hansson, Christer Wingren
      Pages 65-75
  4. Partitioning of Soluble Molecules and Particulates

    1. Front Matter
      Pages 46-75
    2. Jonathan G. Huddleston, Scott T. Griffin, Jianhua Zhang, Heather D. Willauer, Robin D. Rogers
      Pages 77-94
    3. James M. Van Alstine
      Pages 119-142
    4. Lena Hammar
      Pages 143-158
    5. Christer Larsson, Susanne Widell
      Pages 159-166
    6. Hans-Erik Åkerlund
      Pages 167-175
    7. Bengt Jergil, Lars Ekblad
      Pages 193-200
    8. TelmaTeixeira Franco
      Pages 209-218
  5. Isolation and Purification of Proteins by Two-Phase Extraction

    1. Front Matter
      Pages 75-233
    2. Kristina Köhler Van Alstine, Andres Veide
      Pages 251-258
    3. Daniel Forciniti
      Pages 271-280
    4. Anita Kaul, Josefine Persson, Folke Tjerneld
      Pages 281-289
    5. Gerhard Kopperschläger, Jürgen Kirchberger
      Pages 329-344
    6. Roberto Z. Guzmán, Javier E. GarcÍa
      Pages 345-354
    7. Göte Johansson
      Pages 355-361
    8. Gabriel Raya-Tonetti, Nora I. Perotti
      Pages 363-370
    9. Masamichi Kamihira, Rajni Hatti-Kaul, Bo Mattiasson
      Pages 371-379
    10. Teresa Cunha, Raquel Aires-Barros
      Pages 391-409
  6. Bioconversions in Two-Phase Systems

    1. Front Matter
      Pages 235-409
  7. Back Matter
    Pages 427-440

About this book


In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented.
Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Editors and affiliations

  • Rajni Hatti-Kaul
    • 1
  1. 1.Center for Chemistry and Chemical EngineeringLund UniversityLundSweden

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-541-6
  • Online ISBN 978-1-59259-028-5
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site