Animal Cell Biotechnology

Methods and Protocols

  • Nigel Jenkins

Part of the Methods in Biotechnology™ book series (MIBT, volume 8)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Enabling Technologies

    1. Front Matter
      Pages 1-1
    2. Michelle F. Scott, Catherine M. Bentley, Carolynne T. Marshall
      Pages 3-9
    3. Martin S. Sinacore, Denis Drapeau, S. R. Adamson
      Pages 11-22
    4. Alasdair J. Shepherd, Kenneth T. Smith
      Pages 23-36
  3. Molecular Methods

    1. Front Matter
      Pages 37-37
    2. Florian M. Wurm, Dietmar Schiffmann
      Pages 49-59
    3. John Stephen McLean
      Pages 61-72
    4. Margaret Goodall
      Pages 73-98
    5. Stephen J. Froud
      Pages 99-115
  4. Cell Evaluation Protocols

    1. Front Matter
      Pages 129-129
    2. Michael Butler
      Pages 131-144
    3. Afshin Samali, Thomas G. Cotter
      Pages 155-164
    4. Maria L. Anthony, Shane N. O. Williams, Kevin M. Brindle
      Pages 165-175
  5. Specialist Techniques

    1. Front Matter
      Pages 177-177
    2. Malcolm L. Kennard, Gregory A. Lizee, Wilfred A. Jefferies
      Pages 187-200
    3. Malcolm L. Kennard, James M. Piret
      Pages 201-209
    4. Sigma S. Mostafa, Diane L. Hevehan, Todd A. McAdams, E. Terry Papoutsakis, William M. Miller
      Pages 211-227
    5. Todd A. McAdams, E. Terry Papoutsakis, William M. Miller
      Pages 229-238
    6. Lorraine D. Buckberry
      Pages 239-252
  6. Product Evaluation Protocols

    1. Front Matter
      Pages 253-253
    2. Maria G. Castro, Simon Windeatt, Marcelo J. Perone
      Pages 263-275
    3. Andrew D. Hooker, David C. James
      Pages 277-288
    4. Michael J. Gramer
      Pages 289-298
  7. Back Matter
    Pages 299-302

About this book


In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity.
Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.

Editors and affiliations

  • Nigel Jenkins
    • 1
  1. 1.Bioprocess Research and Development Eli Lilly & Co.Indianapolis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-547-8
  • Online ISBN 978-1-59259-486-3
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site