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Quantitative PCR Protocols

  • Bernd Kochanowski
  • Udo Reischl

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 26)

Table of contents

  1. Front Matter
    Pages i-x
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Udo Reischl, Bernd Kochanowski
      Pages 3-30
    3. Luc Raeymaekers
      Pages 31-41
    4. Paul D. Siebert
      Pages 61-85
    5. Olivier Lantz, Elizabeth Bonney, Franck Griscelli, Yassine Taoufik
      Pages 87-102
  3. Protocols

    1. Front Matter
      Pages 117-117
    2. Olivier Lantz, Elizabeth Bonney, Yassine Taoufik
      Pages 157-170
    3. George J. Doellgast, G. Alan Beard, Margaret Sheehan, Nathan Iyer, Louis S. Kucera, Stephen H. Richardson
      Pages 197-216
    4. Anu Suomalainen, Ann-Christine Syvänen
      Pages 233-244
    5. David B. Corry, Richard M. Locksley
      Pages 253-264
    6. Adrian Puntschart, Michael Vogt
      Pages 277-287
    7. Sabine Herblot, Benoît Rousseau, Jacques Bonnet
      Pages 289-300
  4. Back Matter
    Pages 301-304

About this book

Introduction

In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs.
Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.

Editors and affiliations

  • Bernd Kochanowski
    • 1
  • Udo Reischl
    • 1
  1. 1.University of RegensburgGermany

Bibliographic information

  • DOI https://doi.org/10.1385/0896035182
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-518-8
  • Online ISBN 978-1-59259-262-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site