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© 1998

Phospholipid Signaling Protocols

  • Ian M. Bird
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 105)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways

    1. Front Matter
      Pages xiii-xiii
    2. Wendy Bollinger Bollag, Richard D. Griner
      Pages 89-98
    3. Rachel E. Meyers, Lewis C. Cantley
      Pages 99-108
    4. Gregory J. Parker, Joost C. Loijens, Richard A. Anderson
      Pages 127-139
    5. Wendy Bollinger Bollag
      Pages 151-160
    6. Sandie I. Briand, Sylvie G. Bernier, Gaétan Guillemette
      Pages 161-166
    7. Jacob Vadakekalam, Stewart Metz
      Pages 175-185
    8. Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois
      Pages 187-199
    9. Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois
      Pages 201-207

About this book

Introduction

In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry.

Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.

Editors and affiliations

  • Ian M. Bird
    • 1
  1. 1.University of Wisconsin at Madison

Bibliographic information

  • Book Title Phospholipid Signaling Protocols
  • Editors Ian Bird
  • Series Title Methods in Molecular Biology™
  • DOI https://doi.org/10.1385/0896034917
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-491-4
  • Softcover ISBN 978-1-4899-4260-9
  • eBook ISBN 978-1-59259-255-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIII, 380
  • Number of Illustrations 22 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site

Reviews

". . . the detailed information provided in Phospholipid Signaling Protocols makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several protocols in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important protocols together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology