Differential Display Methods and Protocols

  • Peng Liang
  • Arthur B. Pardee

Part of the Methods in Molecular Biology book series (MIMB, volume 85)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Methodologies

    1. Front Matter
      Pages 1-1
    2. Peng Liang, Arthur B. Pardee
      Pages 3-11
    3. Michael McClelland, Rhonda Honeycutt, Francoise Mathieu-Daude, Thomas Vogt, John Welsh
      Pages 13-24
    4. Patrick J. Donohue, Debbie K. W. Hsu, Jeffrey A. Winkles
      Pages 25-35
    5. Takashi Ito, Yoshiyuki Sakaki
      Pages 37-44
    6. Xinkang Wang, Giora Z. Feuerstein
      Pages 69-76
    7. Regina Vögeli-Lange, Niels Bürckert, Thomas Boller, Andres Wiemken
      Pages 95-103
  3. Cloning Family-Specific Genes

  4. Cloning Inducible Genes

    1. Front Matter
      Pages 135-135
    2. Lidia Averboukh, Peng Liang, Stephen A. Douglas, Arthur B. Pardee
      Pages 163-172
  5. Cloning Differentially Expressed Genes During Normal Development

    1. Front Matter
      Pages 173-173
    2. M. Jorge Guimarães, Terrill McClanahan, J. Fernando Bazan
      Pages 175-194
    3. Claudio V. Mello, Erich D. Jarvis, Natalia Denisenko, Miriam Rivas
      Pages 205-217
  6. Cloning Differentially Expressed Genes in Diseases

    1. Front Matter
      Pages 231-231
    2. Mary E. Russell, Anne Räisänen-Sokolowski, Ulrike Utans
      Pages 233-247
    3. William D. Pennie, Yi Sun, Hyungtae Kim, Nancy H. Colburn
      Pages 249-261
    4. Roy A. Jensen, David L. Page, Jeffrey T. Holt
      Pages 277-283
    5. Joseph M. Babity, Richard A. Newton, Mario E. Guido, Harold A. Robertson
      Pages 285-295
    6. Eleftheria Maratos-Flier, Daqing Qu, Steen Gammeltoft
      Pages 297-304
  7. Back Matter
    Pages 305-306

About this book


Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. Also provided are numerous examples-along with detailed experimental procedures-in which differentially expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans.

Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying how gene expression is exactly regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to pathological complications. This novel and productive methodology, fully detailed here, is already playing a major role in the development of selective antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators.

Editors and affiliations

  • Peng Liang
    • 1
  • Arthur B. Pardee
    • 2
  1. 1.Vanderbilt Cancer CenterNashville
  2. 2.Dana-Farber Cancer InstituteBoston

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-489-1
  • Online ISBN 978-1-59259-569-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site