Transmembrane Signaling Protocols

  • Dafna Bar-Sagi

Part of the Methods In Molecular Biology™ book series (MIMB, volume 84)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Overviews

    1. Front Matter
      Pages 1-1
    2. Chi-Hon Lee, David Cowburn, John Kuriyan
      Pages 3-31
    3. Mark A. Lemmon, Joseph Schlessinger
      Pages 49-71
  3. Specific Topics

  4. Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade

    1. Kazuya Shimizu, Toshihisa Ohtsuka, Yoshimi Takai
      Pages 173-183
  5. Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D

  6. Two-Hybrid Analysis of Ras—Raf Interactions

    1. Linda Van Aelst
      Pages 201-222
  7. Cloning and Mutational Analysis of the She-Phosphotyrosine Interaction/ Phosphotyrosine-Binding Domain

  8. Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins

    1. Tyzoon Nomanbhoy, Richard A. Cerione
      Pages 237-247
  9. Prenylation Assays for Small GTPases

    1. Miguel C. Seabra, Guy L. James
      Pages 251-260
  10. Analysis of Myristoylated and Palmitoylated Src Family Proteins

    1. Amy Wolven, Wouter van’t Hof, Marilyn D. Resh
      Pages 261-266
  11. Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded Phospholipid Vesicles

  12. Biochemical and Biological Analyses of Farnesyl-Protein Transferase Inhibitors

    1. Nancy E. Kohl, Kenneth S. Koblan, Charles A. Omer, Allen Oliff, Jackson B. Gibbs
      Pages 283-291
  13. Identification and Characterization of Small GTPase-Associated Kinases

    1. Edward Manser, Thomas Leung, Louis Lim
      Pages 295-305
  14. Functional Studies of Dual-Specificity Phosphatases

  15. Back Matter
    Pages 319-328

About this book


Transmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols.

The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field.

Editors and affiliations

  • Dafna Bar-Sagi
    • 1
  1. 1.State University of New York at Stony BrookNY

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-488-4
  • Online ISBN 978-1-59259-568-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site