Protein Targeting Protocols

  • Roger A. Clegg

Part of the Methods in Molecular Biology™ book series (MIMB, volume 88)

Table of contents

  1. Front Matter
    Pages i-xii
  2. George Panayotou
    Pages 1-10
  3. James G. Pryde
    Pages 23-33
  4. Neil G. Anderson
    Pages 35-45
  5. Zachary E. Hausken, Vincent M. Coghlan, John D. Scott
    Pages 47-64
  6. James Beattie
    Pages 65-69
  7. Victoria E. S. Scott, Christina A. Gurnett, Kevin P. Campbell
    Pages 71-85
  8. Graeme B. Bolger
    Pages 101-131
  9. Michael Csukai, Daria Mochly-Rosen
    Pages 133-139
  10. Grant Scotland, Miles D. Houslay
    Pages 141-150
  11. Morag A. Grassie, Graeme Milligan
    Pages 151-160
  12. Rob Stein
    Pages 187-195
  13. Matthias Frech, Brian A. Hemmings
    Pages 197-210
  14. R. A. Jeffrey McIlhinney
    Pages 211-225
  15. Michael Veit, Michael F. G. Schmidt
    Pages 227-239
  16. Morag A. Grassie, Graeme Milligan
    Pages 241-248
  17. Jean H. Overmeyer, Robert A. Erdman, William A. Maltese
    Pages 249-263

About this book

Introduction

In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored.

Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.




Editors and affiliations

  • Roger A. Clegg
    • 1
  1. 1.Hannah Research InstituteScotlandUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896034879
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-487-7
  • Online ISBN 978-1-59259-572-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book