Polyamine Protocols

  • David M. L. Morgan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 79)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Introduction

    1. David M. L. Morgan
      Pages 3-30
  3. Assay Methods for Enzymes of Polyamine Biosynthesis

    1. Catherine S. Coleman, Anthony E. Pegg
      Pages 41-44
    2. Lisa M. Shantz, Anthony E. Pegg
      Pages 45-50
    3. Laurie Wiest, Anthony E. Pegg
      Pages 51-57
    4. Heather M. Wallace, Deborah M. Evans
      Pages 59-68
    5. Jim Blankenship
      Pages 69-73
  4. Assay Methods for Enzymes of Polyamine Catabolism

    1. Jim Blankenship
      Pages 77-80
    2. R. James Storer, Antonio Ferrante
      Pages 81-90
    3. R. James Storer, Antonio Ferrante
      Pages 91-95
    4. David M. L. Morgan
      Pages 97-103
  5. Measurement of Polyamines

  6. Measurement of Polyamine Transport

    1. David M. L. Morgan
      Pages 139-147
    2. Sarah A. Le Quesne, Alan H. Fairlamb
      Pages 149-156
    3. Heather M. Wallace, A. Jill Mackarel
      Pages 157-165
  7. Measurement of Polyamine Effects on Cell Growth

    1. Christopher P. Denton
      Pages 169-178
  8. Back Matter
    Pages 185-186

About this book


David Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances.

Editors and affiliations

  • David M. L. Morgan
    • 1
  1. 1.King’s CollegeLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-448-8
  • Online ISBN 978-1-59259-565-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site