Table of contents

  1. Front Matter
    Pages i-xi
  2. David R. Higgins, James M. Cregg
    Pages 1-15
  3. James M. Cregg, Shigang Shen, Monique Johnson, Hans R. Waterham
    Pages 17-26
  4. James M. Cregg, Kimberly A. Russell
    Pages 27-39
  5. David R. Higgins, Katie Busser, John Comiskey, Peter S. Whittier, Thomas J. Purcell, James P. Hoeffler
    Pages 41-53
  6. Mike Romanos, Carol Scorer, Koti Sreekrishna, Jeff Clare
    Pages 55-72
  7. David J. Miles, Katie Busser, Christine Stalder, David R. Higgins
    Pages 73-80
  8. Martin A. G. Gleeson, Christopher E. White, David P. Meininger, Elizabeth A. Komives
    Pages 81-94
  9. José A. Cremata, Raquel Montesino, Omar Quintero, Rossana García
    Pages 95-105
  10. Jayne Stratton, Vijay Chiruvolu, Michael Meagher
    Pages 107-120
  11. Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley et al.
    Pages 121-147
  12. Hermann Gram, Rita Schmitz, Rüdiger Ridder
    Pages 179-192
  13. Jeff Clare, Koti Sreekrishna, Mike Romanos
    Pages 193-208
  14. Jeff Clare, Carol Scorer, Rich Buckholz, Mike Romanos
    Pages 209-225
  15. H. Markus Weiß, Winfried Haase, Helmut Reiländer
    Pages 227-239
  16. Winfried Haase, H. Markus Weiß, Helmut Reiländer
    Pages 241-247
  17. Winfried Haase, H. Markus Weiß, Helmut Reiländer
    Pages 249-261
  18. Back Matter
    Pages 263-270

About this book


The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location.

Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.


HIV Peroxisom antibody biology gene gene expression glycoprotein growth membrane molecular biology nucleic acid protein proteins resistance

Editors and affiliations

  • David R. Higgins
    • 1
  • James M. Cregg
    • 2
  1. 1.Chiron Technologies/Center for Gene TherapySan Diego
  2. 2.Oregon Graduate Institute of Science and TechnologyPortland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-421-1
  • Online ISBN 978-1-59259-578-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site