Lectin Methods and Protocols

  • Jonathan M. Rhodes
  • Jeremy D. Milton

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 9)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Lectin Histochemistry and Cytochemistry

    1. Front Matter
      Pages 1-1
    2. Anthony J. Leathem, Susan A. Brooks
      Pages 3-20
    3. Robert W. Stoddart, Carolyn J. P. Jones
      Pages 21-39
    4. Udo Schumacher, Barry S. Mitchell
      Pages 55-64
    5. Adi Ellinger
      Pages 97-110
    6. Rainer Herken, Berti Manshausen
      Pages 111-119
    7. Juan F. Madrld, Francisco Hernández, José Ballesta
      Pages 121-132
  3. Use of Lectins for Structural Analysis of Oligosaccharide Chains

    1. Front Matter
      Pages 145-145
    2. Gregers J. Gram, John-Erik Stig Hansen
      Pages 167-174
  4. Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins

    1. Front Matter
      Pages 193-193
    2. Naoyuki Tsuchiya, Tamao Endo, Naohisa Kochibe, Koji Ito, Akira Kobata
      Pages 195-205
    3. Taei Matsui, Koiti Titani
      Pages 235-245
  5. Use of Lectins in Quantification of Soluble Glycoproteins

    1. Front Matter
      Pages 247-247
    2. Jeremy.D. Milton, Jonathan M. Rhodes
      Pages 255-261
  6. Lectins in Affinity Purification of Soluable Glycoproteins

    1. Front Matter
      Pages 263-263
    2. Michael A. Kerr, Lesley M. Loomes, Brian C. Bonner, Amy B. Hutchings, Bernard W. Senior
      Pages 265-278
    3. Kelly P. Kearse
      Pages 291-298
  7. Lectins in Flow Cytometry

  8. Lectins as Tools for Cell Purification/Purging

  9. Effects of Lectins on Mammalian Cells

    1. Front Matter
      Pages 363-363
    2. David C. Kilpatrick
      Pages 365-378
    3. Lu-Gang Yu, Jonathan M. Rhodes
      Pages 379-384
    4. David C. Kilpatrick
      Pages 385-392
    5. Susan Bardocz, Ann White
      Pages 393-405
    6. Paolo Carinci, Ennio Becchetti, Maria Bodo
      Pages 407-421
    7. Kiyonao Sada, Hirohei Yamamura
      Pages 423-432
    8. Giuseppe Ramaschi, Mauro Torti
      Pages 433-439
    9. Alexander V. Timoshenko, Klaus Kayser, Hans-Joachim Gabius
      Pages 441-451
    10. Elieser Gorelik
      Pages 453-459
  10. Effects of Lectins in Organ Culture

    1. Front Matter
      Pages 473-473

About this book


Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology

Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. The book will immediately become the standard practical resource and guide to lectin techniques for today's biomedical researchers.

Editors and affiliations

  • Jonathan M. Rhodes
    • 1
  • Jeremy D. Milton
    • 1
  1. 1.University of LiverpoolUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-396-2
  • Online ISBN 978-1-59259-593-8
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site