Flow Cytometry Protocols

  • Mark J. Jaroszeski
  • Richard Heller

Part of the Methods in Molecular Biology™ book series (MIMB, volume 91)

Table of contents

  1. Front Matter
    Pages i-x
  2. Gilbert Radcliff, Mark J. Jaroszeski
    Pages 1-24
  3. Elisabeth Paietta
    Pages 25-36
  4. Raymond H. Widen, Jeanne L. Becker
    Pages 57-65
  5. Adorjan Aszalos, James L. Weaver
    Pages 117-122
  6. mnKhalid El Ouagari, Justin Teissié
    Pages 133-139
  7. Marie-Pierre Rols, Justin Teissié
    Pages 141-147
  8. Mark J. Jaroszeski, Richard Gilbert, Richard Heller
    Pages 149-156
  9. Michael G. Ormerod
    Pages 157-165
  10. Kim Steck, Adel El-Naggar
    Pages 181-195
  11. Kenneth J. Pennline
    Pages 197-215
  12. Wojclech Gorczyca, Myron R. Melamed, Zbigniew Darzynkiewicz
    Pages 217-238
  13. Simon P. Monard
    Pages 239-254
  14. Kenneth J. Pennline
    Pages 255-269
  15. Back Matter
    Pages 271-274

About this book


In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed.

Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology.

Editors and affiliations

  • Mark J. Jaroszeski
    • 1
  • Richard Heller
    • 1
  1. 1.University of South FloridaTampa

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 1998
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-354-2
  • Online ISBN 978-1-59259-214-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site