Protein Sequencing Protocols

  • Bryan John Smith

Part of the Methods in Molecular Biology™ book series (MIMB, volume 64)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Bryan John Smith, Paul Tempst
    Pages 1-16
  3. Bryan John Smith, Colin Wheeler
    Pages 43-55
  4. Bryan John Smith
    Pages 57-72
  5. Chris W. Sutton, Jacqui A. O’Neill
    Pages 73-79
  6. Tomoko Hayashi Akiyama, Tatsuru Sasagawa
    Pages 81-83
  7. Tomoko Hayashi Akiyama, Tatsuru Sasagawa
    Pages 85-89
  8. G. Brent Irvine
    Pages 131-138
  9. Alan J. Smith
    Pages 139-146
  10. Rong Wang, Brian T. Chait
    Pages 175-182
  11. Gregory A. Grant, Mark W. Crankshaw
    Pages 197-215
  12. Tomoko Hayashi Akiyama, Tatsuru Sasagawa
    Pages 235-242
  13. Franca Casagranda, John F. K. Wilshire
    Pages 243-257
  14. Jerome M. Bailey, Chad G. Miller
    Pages 259-269
  15. Alex F. Carne, Sally U
    Pages 271-284
  16. Hisashi Hirano, Setsuko Komatsu, Susumu Tsunasawa
    Pages 285-292
  17. Alastair Aitken, Michèle Learmonth
    Pages 293-306
  18. Chris W. Sutton, Jacqui A. O’Neill
    Pages 307-315
  19. Alastair Aitken, Michèle Learmonth
    Pages 317-328

About this book


Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples.

Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.

Editors and affiliations

  • Bryan John Smith
    • 1
  1. 1.Celltech TherapeuticsSloughUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-353-5
  • Online ISBN 978-1-59259-550-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site