Protocols for Nucleic Acid Analysis by Nonradioactive Probes

  • Peter G. Isaac

Part of the Methods in Molecular Biology™ book series (MIMB, volume 28)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Justin Stacey, Peter G. Isaac
    Pages 9-15
  3. Rachel Hodge
    Pages 37-40
  4. Rachel Hodge
    Pages 49-54
  5. Rachel Hodge
    Pages 55-57
  6. Peter G. Isaac, Justin Stacey
    Pages 59-66
  7. Ian Durrant, Timothy Stone
    Pages 89-92
  8. Bronwen M. Harvey, Claire B. Wheeler, Martin W. Cunningham
    Pages 93-100
  9. Patricia M. E. Chadwick, Ian Durrant
    Pages 101-105
  10. Elizabeth Davies, Rachel Hodge, Peter G. Isaac
    Pages 121-125

About this book

Introduction

In assembling this book, Protocols for Nucleic Acid Analysis by Nonradioactive Probes, I have endeavored to select protocols that have wide applicability. My aim in doing so is to allow nucleic acid analysis to move from the confines of specialized research labora- ries and into general purpose and teaching laboratories. In particular, disciplines such as population biology and clinical diagnostics (where the application of the technology is important, not the technology itself) will find the newer techniques easier to perform with the qu- tity of samples that are normally required in these fields. Die-hard molecular biologists with hot fingers will find, by trying some of the protocols in this book, that nonradioactive protocols are normally faster than their radioactive brethren, and give more reliable results. An expanded description of the subject areas covered by this book is given in Chapter 1. The technology herein has very broad application; for instance, in conventional molecular biology research, population biology, plant and animal breeding, genetic mapping, paternity testing, forensics, prenatal diagnosis, and clinical and food microbiology. I have tried to include comprehensive protocols for both basic and more complex analyses. There are chapters dealing with the fundamentals of nucleic acid extraction from plants and a- mals, and with the procedures necessary to immobilize nucleic acids on solid supports. A range of labeling procedures is included, f- lowed by a selection of hybridization procedures.

Editors and affiliations

  • Peter G. Isaac
    • 1
  1. 1.Nickerson BIOCEM Ltd.CambridgeUK

Bibliographic information

  • DOI https://doi.org/10.1385/089603254X
  • Copyright Information Humana Press 1994
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-254-5
  • Online ISBN 978-1-59259-515-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book