Practical Cell Culture Techniques

  • Alan A. Boulton
  • Glen B. Baker
  • Wolfgang Walz

Part of the Neuromethods book series (NM, volume 23)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Diane E. Harold, Wolfgang Walz
    Pages 1-20
  3. Colin J. Barnstable
    Pages 21-62
  4. Jane E. Bottenstein
    Pages 63-85
  5. Marston Manthorpe, David Muir, Brigitte Pettmann, Silvio Varon
    Pages 87-137
  6. Daren Ure, Ann Acheson
    Pages 139-171
  7. B. Rogister, J. M. Rigo, P.P. Lefebvre, P. Leprince, P. Delree, D. Martin et al.
    Pages 173-200
  8. Jeffrey R. Buchhalter, Marc A. Dichter
    Pages 241-268
  9. Bernhard H. J. Juurlink, Leif Hertz
    Pages 269-321
  10. Jean de Vellis, Araceli Espinosa de los Monteros
    Pages 323-352
  11. Nika V. Ketis
    Pages 353-378
  12. Back Matter
    Pages 379-392

About this book


Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Editors and affiliations

  • Alan A. Boulton
    • 1
  • Glen B. Baker
    • 2
  • Wolfgang Walz
    • 3
  1. 1.University of SaskatchewanSaskatoonCanada
  2. 2.University of AlbertaEdmontonCanada
  3. 3.University of SaskatchewanSaskatoonCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1992
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-214-9
  • Online ISBN 978-1-59259-628-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site