New Protein Techniques Editors (view affiliations) John M. Walker Book 47 Citations 1 Mentions 158 Readers 32k Downloads Part of the Methods in Molecular Biology™ book series (MIMB, volume 3)
Protocols Table of contents (38 protocols) About About this book Table of contents Page 1 Navigate to page number of 2 Next Navigate to page number Search within book Front Matter Pages i-xv PDF Prevention of Unwanted Proteolysis Robert J. Beynon Pages 1-23 The Bradford Method for Protein Quantitation John B. W. Hammond, Nicholas J. Kruger Pages 25-32 Amino Acid Analysis by Precolumn Derivatization E. L. V. Harris Pages 33-47 Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography E. L. V. Harris Pages 49-55 Enzymatic Methods for Cleaving Proteins Bryan John Smith Pages 57-69 Chemical Cleavage of Proteins Bryan John Smith Pages 71-88 Chemical Modification of Proteins Alex F. Carne Pages 89-98 The Design, Preparation, and Use of Immunopurification Reagents Andrew Kenney, Lynne Goulding, Christopher Hill Pages 99-110 Dye-Ligand Chromatography Sarojani Angal Pages 111-122 Aminohexyl-Sepharose Affinity Chromatography R. D. J. Barker, H. M. Bailey Pages 123-134 Purification of DNA-Dependent RNA Polymerase from Eubacteria N. W. Scott Pages 135-147 Direct Immunoprecipitation of Protein Christopher F. Thurston, Lucy F. Henley Pages 149-158 Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique Ketan Patel, David J. Easty, Michael J. Dunn Pages 159-168 Chromatofocusing Ake Sidén, Paolo Gallo Pages 169-186 Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels Michael J. Dunn, Ketan Patel Pages 187-201 Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension Michael J. Dunn, Ketan Patel Pages 203-216 Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension Michael J. Dunn, Ketan Patel Pages 217-231 Preparative Aspects of Immobilized pH Gradients Pier Giorgio Righetti, Cecilia Gelfi Pages 233-256 Isoelectric Focusing Under Denaturing Conditions Christopher F. Thurston, Lucy F. Henley Pages 257-267 Page 1 Navigate to page number of 2 Next About this book Editors and affiliations John M. Walker11.The Hatfield PolytechnicHatfieldUK Bibliographic information DOI https://doi.org/10.1385/0896031268 Copyright Information Humana Press 1988 Publisher Name Humana Press eBook Packages Springer Protocols Print ISBN 978-0-89603-126-5 Online ISBN 978-1-59259-490-0 Series Print ISSN 1064-3745 Series Online ISSN 1940-6029 About this book Buy options