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New Protein Techniques

  • John M. Walker
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 3)

Table of contents

  1. Front Matter
    Pages i-xv
    PDF
  2. Prevention of Unwanted Proteolysis
    Robert J. Beynon
    Pages 1-23
  3. The Bradford Method for Protein Quantitation
    John B. W. Hammond, Nicholas J. Kruger
    Pages 25-32
  4. Amino Acid Analysis by Precolumn Derivatization
    E. L. V. Harris
    Pages 33-47
  5. Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography
    E. L. V. Harris
    Pages 49-55
  6. Enzymatic Methods for Cleaving Proteins
    Bryan John Smith
    Pages 57-69
  7. Chemical Cleavage of Proteins
    Bryan John Smith
    Pages 71-88
  8. Chemical Modification of Proteins
    Alex F. Carne
    Pages 89-98
  9. The Design, Preparation, and Use of Immunopurification Reagents
    Andrew Kenney, Lynne Goulding, Christopher Hill
    Pages 99-110
  10. Dye-Ligand Chromatography
    Sarojani Angal
    Pages 111-122
  11. Aminohexyl-Sepharose Affinity Chromatography
    R. D. J. Barker, H. M. Bailey
    Pages 123-134
  12. Purification of DNA-Dependent RNA Polymerase from Eubacteria
    N. W. Scott
    Pages 135-147
  13. Direct Immunoprecipitation of Protein
    Christopher F. Thurston, Lucy F. Henley
    Pages 149-158
  14. Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
    Ketan Patel, David J. Easty, Michael J. Dunn
    Pages 159-168
  15. Chromatofocusing
    Ake Sidén, Paolo Gallo
    Pages 169-186
  16. Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels
    Michael J. Dunn, Ketan Patel
    Pages 187-201
  17. Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension
    Michael J. Dunn, Ketan Patel
    Pages 203-216
  18. Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
    Michael J. Dunn, Ketan Patel
    Pages 217-231
  19. Preparative Aspects of Immobilized pH Gradients
    Pier Giorgio Righetti, Cecilia Gelfi
    Pages 233-256
  20. Isoelectric Focusing Under Denaturing Conditions
    Christopher F. Thurston, Lucy F. Henley
    Pages 257-267
  21. Computer Analysis of 2-D Electrophoresis Gels
    H. H-S. Ip
    Pages 269-298
  22. Two-Dimensional (Crossed) Immunoelectrophoresis
    John M. Walker
    Pages 299-310
  23. Peptide Synthesis
    Brian Austen
    Pages 311-331
  24. Synthesis of a Series of Analogous Peptides Using T-Bags
    Brian Austen
    Pages 333-339
  25. Production of Antisera to Synthetic Peptides
    W. J. Gullick
    Pages 341-354
  26. Production of Antibodies Using Proteins in Gel Bands
    S. A. Amero, T. C. James, S. C. R. Elgin
    Pages 355-362
  27. Purification of Immunoglobulins Using Protein A-Sepharose
    Nicholas J. Kruger, John B. W. Hammond
    Pages 363-371
  28. Antibody-Enzyme Conjugate Formation
    G. B. Wisdom
    Pages 373-382
  29. Vacuum Blotting
    Marnix Peferoen
    Pages 383-393
  30. Blotting with Plate Electrodes
    Marnix Peferoen
    Pages 395-402
  31. Use of Dried Milk for Immunoblotting
    Rosemary Jagus, Jeffrey W. Pollard
    Pages 403-408
  32. Immunodetection of Proteins on “Western” Blots Using 125I-Labeled Protein A
    Nicholas J. Kruger, John B. W. Hammond
    Pages 409-417
  33. Detection of Protein Blots Using the Avidin-Biotin System
    Michael J. Dunn, Ketan Patel
    Pages 419-426
  34. Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A
    J. M. Walker, W. Gaastra
    Pages 427-440
  35. Colloidal Gold for the Detection of Proteins on Blots and Immunoblots
    Alan Jones, Marc Moeremans
    Pages 441-479
  36. Enzyme Immobilization by Adsorption
    M. D. Trevan
    Pages 481-489
  37. Enzyme Immobilization by Entrapment
    M. D. Trevan
    Pages 491-494
  38. Enzyme Immobilization by Covalent Bonding
    M. D. Trevan
    Pages 495-510
  39. Cell Immobilization
    M. D. Trevan
    Pages 511-523
  40. Back Matter
    Pages 525-531
    PDF

About this book

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.The Hatfield PolytechnicHatfieldUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896031268
  • Copyright Information Humana Press 1988
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-126-5
  • Online ISBN 978-1-59259-490-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site