New Protein Techniques

  • John M. Walker
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 3)

Table of contents

  1. Front Matter
    Pages i-xv
    PDF
  2. Prevention of Unwanted Proteolysis
    Robert J. Beynon
    Pages 1-23
  3. The Bradford Method for Protein Quantitation
    John B. W. Hammond, Nicholas J. Kruger
    Pages 25-32
  4. Amino Acid Analysis by Precolumn Derivatization
    E. L. V. Harris
    Pages 33-47
  5. Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography
    E. L. V. Harris
    Pages 49-55
  6. Enzymatic Methods for Cleaving Proteins
    Bryan John Smith
    Pages 57-69
  7. Chemical Cleavage of Proteins
    Bryan John Smith
    Pages 71-88
  8. Chemical Modification of Proteins
    Alex F. Carne
    Pages 89-98
  9. The Design, Preparation, and Use of Immunopurification Reagents
    Andrew Kenney, Lynne Goulding, Christopher Hill
    Pages 99-110
  10. Dye-Ligand Chromatography
    Sarojani Angal
    Pages 111-122
  11. Aminohexyl-Sepharose Affinity Chromatography
    R. D. J. Barker, H. M. Bailey
    Pages 123-134
  12. Purification of DNA-Dependent RNA Polymerase from Eubacteria
    N. W. Scott
    Pages 135-147
  13. Direct Immunoprecipitation of Protein
    Christopher F. Thurston, Lucy F. Henley
    Pages 149-158
  14. Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
    Ketan Patel, David J. Easty, Michael J. Dunn
    Pages 159-168
  15. Chromatofocusing
    Ake Sidén, Paolo Gallo
    Pages 169-186
  16. Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels
    Michael J. Dunn, Ketan Patel
    Pages 187-201
  17. Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension
    Michael J. Dunn, Ketan Patel
    Pages 203-216
  18. Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
    Michael J. Dunn, Ketan Patel
    Pages 217-231
  19. Preparative Aspects of Immobilized pH Gradients
    Pier Giorgio Righetti, Cecilia Gelfi
    Pages 233-256
  20. Isoelectric Focusing Under Denaturing Conditions
    Christopher F. Thurston, Lucy F. Henley
    Pages 257-267

About this book

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.The Hatfield PolytechnicHatfieldUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896031268
  • Copyright Information Humana Press 1988
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-126-5
  • Online ISBN 978-1-59259-490-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book