Protein Analysis and Purification

1. Front Matter

   Pages i-xxvi

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2. An Overview of This Manual

   Pages 1-7

3. Protein Structure

   Pages 8-23

4. Tracking the Target Protein

   Pages 24-62

5. Electrophoretic Techniques

   Pages 63-117

6. Getting Started with Protein Purification

   Pages 118-152

7. Membrane Proteins

   Pages 153-176

8. Transfer and Detection of Proteins on Membrane Supports

   Pages 177-210

9. Identification of the Target Protein

   Pages 211-258

10. Identifying and Analyzing Posttranslational Modifications

    Pages 259-323

11. Chromatography

    Pages 324-384

12. Recombinant Protein Techniques

    Pages 385-430

13. Back Matter

    Pages 431-520

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How one goes about analyzing proteins is a constantly evolving ?eld that is no longer solely the domain of the protein biochemist. Inves- gators from diverse disciplines ?nd themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are ?lled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to ?t individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scienti?c research is performed. Bioinformatics and In silico science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.

• Analysis
• Benchtop
• Protein
• Purification
• Rosenberg
• Techniques
• Translation
• membrane proteins
• protein structure

• G.I. Unit, Massachusetts General Hospital, Boston, USA

  Ian M. Rosenberg

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