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Doubled Haploid Production in Crop Plants

A Manual

  • Book
  • © 2003

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Editors:
  1. M. Maluszynski

    1. Joint FAO/IAEA Division of Nuclear Technique in Food and Agriculture, Vienna, Austria

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  2. K. J. Kasha

    1. Department of Plant Agriculture, University of Guelph, Guelph, Canada

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  3. B. P. Forster

    1. Scottish Crop Research Institute, Dundee, UK

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  4. I. Szarejko

    1. Department of Genetics, University of Silesia, Katowice, Poland

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About this book

The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.

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Table of contents (54 chapters)

  1. Front Matter

    Pages N1-xlvi
    Download chapter PDF

  2. Production of doubled haploids in crop plants. An introduction

    1. Production of doubled haploids in crop plants. An introduction

      • K. J. Kasha, M. Maluszynski
      Pages 1-4

  3. Protocols for major crops

    1. Barley

      1. Doubled haploid production in barley using the <i>Hordeum bulbosum</i> (L.) technique
        • P. Hayes, A. Corey, J. DeNoma
        Pages 5-14
      2. The <i>Hordeum bulbosum</i> (L.) method
        • P. Devaux
        Pages 15-19
      3. Anther culture in barley
        • C. Jacquard, G. Wojnarowiez, C. Clément
        Pages 21-27
      4. Barley anther culture
        • L. Cistué, M. P. Vallés, B. Echávarri, J. M. Sanz, A. Castillo
        Pages 29-34
      5. Anther culture for doubled haploid production in barley (<i>Hordeum vulgare</i> L.)
        • I. Szarejko
        Pages 35-42
      6. Barley isolated microspore culture protocol
        • K. J. Kasha, E. Simion, R. Oro, Y. S. Shim
        Pages 43-47
      7. Barley isolated microspore culture (IMC) method
        • P. A. Davies
        Pages 49-52

    2. Wheat

      1. Doubled haploid production in wheat through wide hybridization
        • M. N. Inagaki
        Pages 53-58
      2. Protocol for wheat (<i>Triticum aestivum</i> L.) anther culture
        • J. Pauk, R. Mihály, M. Puolimatka
        Pages 59-64
      3. Protocol for producing doubled haploid plants from anther culture of wheat (<i>Triticum aestivum</i> L.<i>)</i>
        • B. Barnabás
        Pages 65-70
      4. Wheat anther culture
        • S. Tuvesson, R. von Post, A. Ljungberg
        Pages 71-76
      5. Haploid wheat isolated microspore culture protocol
        • K. J. Kasha, E. Simion, M. Miner, J. Letarte, T. C. Hu
        Pages 77-81
      6. Production of doubled haploids in wheat (<i>Triticum aestivum</i> L.) through microspore embryogenesis triggered by inducer chemicals
        • M. Y. Zheng, W. Liu, Y. Weng, E. Polle, C. F. Konzak
        Pages 83-94

    4. Rice indica/japanica

      1. Laboratory protocol for anther culture technique in rice
        • F. J. Zapata-Arias
        Pages 109-116

    5. Triticale

      1. Triticale anther culture
        • S. Tuvesson, R. von Post, A. Ljungberg
        Pages 117-121
      2. Protocol for anther culture in hexaploid triticale (x <i>Triticosecale</i> Wittm.)
        • M. WÄ™dzony
        Pages 123-128
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Editors and Affiliations

  • Joint FAO/IAEA Division of Nuclear Technique in Food and Agriculture, Vienna, Austria

    M. Maluszynski

  • Department of Plant Agriculture, University of Guelph, Guelph, Canada

    K. J. Kasha

  • Scottish Crop Research Institute, Dundee, UK

    B. P. Forster

  • Department of Genetics, University of Silesia, Katowice, Poland

    I. Szarejko

Bibliographic Information

  • Book Title: Doubled Haploid Production in Crop Plants

  • Book Subtitle: A Manual

  • Editors: M. Maluszynski, K. J. Kasha, B. P. Forster, I. Szarejko

  • DOI: https://doi.org/10.1007/978-94-017-1293-4

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-1-4020-1544-1Published: 30 November 2003

  • Softcover ISBN: 978-90-481-6393-9Published: 12 December 2011

  • eBook ISBN: 978-94-017-1293-4Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XLVI, 428

  • Number of Illustrations: 23 b/w illustrations, 239 illustrations in colour

  • Topics: Plant Sciences, Agriculture, Tree Biology, Life Sciences, general

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