Animal Cell Technology: Basic & Applied Aspects

1. Effect of various substances on animal cell response

   1. Cytotoxicity Testing of Tomatine

      • Daio Kozuka, Shiro Yamashoji, Yukiko Chiba, Kenji Isshiki

      Pages 115-119

   2. Mannosylerythritol Lipid Induced Differentiation of Human Myelogenous Leukemia Cell Line K-562

      • Hiroko Isoda, Hiroshi Shinmoto, Dai Kitamoto, Masatoshi Matsumura, Tadaatsu Nakahara

      Pages 121-125

   3. Coffee’s Inhibitory Action on the Invasion of Hepatoma Cells Co-Cultured with Mesothelial Cells

      • T. Furuse, H. Shiomi, Y. Miura, K. Yagasaki

      Pages 127-130

   4. Inhibitory Effects of Tea Catechins on Invasion of Human Fibrosarcoma HT1080 Cells to the Monolayer of Human Umbilical Vein Endothelial Cells

      • Mari Maeda-Yamamoto, Kazuhiro Osada, Yuichi Yamaguchi, Kenkou Tsuji

      Pages 131-135

   5. Norzoanthamine Suppresses PTH-Stimulated IL-6 Production in Vitro and Bone Resorption in Vivo

      • Tomoko Tsuji, Kohji Yamaguchi, Mika Yada, Kaoru Yamada, Daisuke Uemura

      Pages 137-141

   6. Preparation and Biological Activities of High Molecular Weight Substances from Dried Safflower Petals

      • Tetsuya Wakabayashi, Takao Kataoka, Je-Tae Woo, Kazuo Nagai

      Pages 143-148

2. Basics and applications in Immunology

   1. Characterization of Human T Cell Clones Isolated from Immortalized T Cell Lines Generated by Oncogene Transfection

      • Shahabuddin Alam, Yoshinori Katakura, Sanetaka Shirahata

      Pages 149-153

   2. Acquired Autoantibodies from Human Plasma B Cells by Light Chain Shifting

      • Siripen Thongpassano, Sanetaka Shirahata, Hirofumi Tachibana

      Pages 155-159

   3. Clinical Application of Anti-Carcinoma Human Monoclonal Antibody

      • Keiji Koda, Mark C. Glassy, Nobuyuki Nakajima

      Pages 161-164

   4. Some Properties of Bispecific Antibodies to Bovine Lactoferrin and Horseradish Peroxidase Secreted by a Hybrid-Hybridoma HH1-4-3

      • Hiroshi Shinmoto, Masuko Kobori, Tojiro Tsushida, Kazuki Shinohara

      Pages 165-169

   5. Development of an Elisa Based Assay for Measuring Glycosyltransferase Activity Using Synthetic Glycopolymers

      • Mohamed Oubihi, Ken Kitajima, Kazukiyo Kobayashi, Takahiro Adachi, Naohito Aoki, Tsukasa Matsuda

      Pages 171-176

   6. Concanamycin A, a Vacuolar Type H+-Atpase Inhibitor, Selectively Induces Cell Death in Activated CD8+ CTL

      • Ken-Ichi Togashi, Takao Kataoka, Kazuo Nagai

      Pages 177-182

3. Technology and engineering for cell culture

   1. Yield Improvements Utilising Fed-Batch Cultivation and Optimisation Strategies

      • C. S. Sanderson, C. P. Marquis, J. P. Barford

      Pages 183-189

   2. Quality Aspects of Batch Animal Cell Cultivation — Relevance to Fed-Batch Implementation in Industry

      • D. J. Black, J. P. Barford, C. Harbour

      Pages 191-196

   3. Technical Approaches to Minimize Regulatory Hurdles to Fed-Batch and Continuous Medium Supplementation

      • David W. Jayme, Richard M. Fike, Stephen F. Gorfien, Glenn P. Godwin

      Pages 197-201

   4. The Use Of Biosensors in Control of Fed-Batch Processes — Opportunities and Limitations

      • R. Renneberg, J. P. Barford, S. M. Tse

      Pages 203-210

   5. Metabolic System Engineering Approach for Saccharomyces cerevisiae and Hybridoma Cell Culture

      • Kaiming Ye, Huidong Shi, Kazuyuki Shimizu

      Pages 211-216

   6. Adaptive Fuzzy Control of Mammalian Cell Culture in Fed-Batch Reactor for Production of an Antithrombin III Variant

      • Petros Lenas, Tamotsu Kitade, Hideki Watanabe, Hiroyuki Honda, Takeshi Kobayashi

      Pages 217-221

   7. Cost-Saving Design and Operational Options for Large-Scale Production of Nutrient Medium and Buffers

      • David Jayme, James Kubiak, Richard Fike, Stephan Rashbaum, Shawn Smith

      Pages 223-227

   8. Effect of Shear Stress on the rCHO Cells

      • M. Motobu, P. C. Wang, M. Matsumura

      Pages 229-233

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.

• biotechnology
• cell biology
• development
• gene therapy
• protein
• proteins

• Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan

  K. Nagai, M. Wachi

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