Fundamentals of Fluorescence Microscopy

Exploring Life with Light

  • Partha Pratim Mondal
  • Alberto Diaspro

Table of contents

  1. Front Matter
    Pages I-XV
  2. Basics and Fundamentals

    1. Front Matter
      Pages 1-1
    2. Partha Pratim Mondal, Alberto Diaspro
      Pages 3-31
    3. Partha Pratim Mondal, Alberto Diaspro
      Pages 33-51
    4. Partha Pratim Mondal, Alberto Diaspro
      Pages 53-70
    5. Partha Pratim Mondal, Alberto Diaspro
      Pages 71-94
    6. Partha Pratim Mondal, Alberto Diaspro
      Pages 95-107
  3. Advanced Imaging

    1. Front Matter
      Pages 109-109
    2. Partha Pratim Mondal, Alberto Diaspro
      Pages 111-134
    3. Partha Pratim Mondal, Alberto Diaspro
      Pages 135-147
    4. Partha Pratim Mondal, Alberto Diaspro
      Pages 149-159
    5. Partha Pratim Mondal, Alberto Diaspro
      Pages 161-187
    6. Partha Pratim Mondal, Alberto Diaspro
      Pages 189-202
    7. Partha Pratim Mondal, Alberto Diaspro
      Pages 203-207
  4. Back Matter
    Pages 209-218

About this book

Introduction

This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors.

The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence  factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices.

The book is organized into two parts. The first part deals with basics of imaging optics and its applications.  The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.

Keywords

Advanced fluorescence microscopy techniques Advances in fluorescence spectroscopy and microscopy Fluorophores and fluorescent probes Fourier, Fresnel, Fraunhofer geometrical optics Imaging optics Molecular physics and photophysics Quantitative fluorescence microscopy Ray optics and wave optics Super-resolution microscopy Textbook on fluorescence imaging

Authors and affiliations

  • Partha Pratim Mondal
    • 1
  • Alberto Diaspro
    • 2
  1. 1.Dept. Instrumentation and Applied PhysicsIndian Institute of ScienceBangaloreIndia
  2. 2.Nanophysics UnitThe Italian Institute of Technology - IIGenovaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-007-7545-9
  • Copyright Information Springer Science+Business Media Dordrecht 2014
  • Publisher Name Springer, Dordrecht
  • eBook Packages Physics and Astronomy
  • Print ISBN 978-94-007-7544-2
  • Online ISBN 978-94-007-7545-9
  • About this book