© 2015

Targeted Genome Editing Using Site-Specific Nucleases

ZFNs, TALENs, and the CRISPR/Cas9 System

  • Takashi Yamamoto

Table of contents

  1. Front Matter
    Pages i-viii
  2. Basics of Genome Editing

  3. Genome Editing in Cultured Cells and Various Organisms

    1. Front Matter
      Pages 43-43
    2. Hongmei Lisa Li, Akitsu Hotta
      Pages 45-69
    3. Takuma Sugi
      Pages 71-80
    4. Naoaki Sakamoto
      Pages 97-106
    5. Nicholas Treen, Yasunori Sasakura
      Pages 107-117
    6. Atsuo Kawahara, Taijiro Yabe, Satoshi Ansai, Shinji Takada, Masato Kinoshita
      Pages 119-131
    7. Ken-ichi T. Suzuki, Toshinori Hayashi
      Pages 133-149
    8. Samantha A. M. Young, Mark Baker, Masahito Ikawa
      Pages 151-166
    9. Tomomi Aida
      Pages 167-182
    10. Kazuto Yoshimi, Takehito Kaneko, Birger Voigt, Tomoji Mashimo
      Pages 183-195
    11. Yuriko Osakabe, Keishi Osakabe
      Pages 197-205

About this book


This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.


CRISPR/Cas9 Gene disruption Gene knockout Genome editing Knock-in TALEN Targeted insertion ZFN iPS

Editors and affiliations

  • Takashi Yamamoto
    • 1
  1. 1.Department of Mathematical and Life SciencesHiroshima UniversityHigashi-HiroshimaJapan

Bibliographic information