Overview
- Equips readers with a basic understanding of genome editing methods, allowing for further developments in the field
- Provides detailed methods for generation of engineered nucleases
- Describes applications of genome editing methods in various organisms and in stem cells
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About this book
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
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Keywords
Table of contents (13 chapters)
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Front Matter
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Genome Editing in Cultured Cells and Various Organisms
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Targeted Genome Editing Using Site-Specific Nucleases
Book Subtitle: ZFNs, TALENs, and the CRISPR/Cas9 System
Editors: Takashi Yamamoto
DOI: https://doi.org/10.1007/978-4-431-55227-7
Publisher: Springer Tokyo
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Japan 2015
Hardcover ISBN: 978-4-431-55226-0Published: 20 January 2015
Softcover ISBN: 978-4-431-56235-1Published: 06 October 2016
eBook ISBN: 978-4-431-55227-7Published: 05 January 2015
Edition Number: 1
Number of Pages: VIII, 205
Number of Illustrations: 12 b/w illustrations, 33 illustrations in colour
Topics: Transgenics, Developmental Biology, Cell Biology, Animal Genetics and Genomics, Plant Genetics and Genomics