© 2017

Hydrocarbon and Lipid Microbiology Protocols

Bioproducts, Biofuels, Biocatalysts and Facilitating Tools

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales
  • Offers readily reproducible, step-by-step laboratory methods

  • Provides helpful tips and tricks to complement the protocols

  • Includes troubleshooting advice

  • Features practical laboratory guidelines to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mónica Martínez-Martínez, Peter N. Golyshin, Manuel Ferrer
    Pages 13-36
  3. Thomas Classen, Filip Kovacic, Benjamin Lauinger, Jörg Pietruszka, Karl-Erich Jaeger
    Pages 37-69
  4. Iain D. Hay, David O. Hooks, Bernd H. A. Rehm
    Pages 71-86
  5. O. Revelles, I. Calvillo, A. Prieto, M. A. Prieto
    Pages 105-119
  6. William A. Muzika, Nymul E. Khan, Lauren M. Jackson, Nicholas Winograd, Wayne R. Curtis
    Pages 141-152
  7. Jean-Marc Nicaud, Anne-Marie Crutz-Le Coq, Tristan Rossignol, Nicolas Morin
    Pages 153-169
  8. A. Schnürer, I. Bohn, J. Moestedt
    Pages 171-200

About this book


This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Biocorrosion Biosurfactant production Fuel quality Hydrocarbon degradation Isoprenoid biosynthesis

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of Essex Dept. Biological SciencesColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Bioproducts, Biofuels, Biocatalysts and Facilitating Tools
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-53113-6
  • Softcover ISBN 978-3-662-57114-9
  • eBook ISBN 978-3-662-53115-0
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 245
  • Number of Illustrations 20 b/w illustrations, 30 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site