Overview
- Offers readily-reproducible step-by-step laboratory methods
- Provides helpful tips and tricks supporting the protocols
- Includes troubleshooting advice
- Contains practical laboratory guidance to promote successful results
Part of the book series: Springer Protocols Handbooks (SPH)
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About this book
This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
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Keywords
Table of contents (11 protocols)
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Front Matter
Editors and Affiliations
Bibliographic Information
Book Title: Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle: Synthetic and Systems Biology - Tools
Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-662-50432-1
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag Berlin Heidelberg 2016
Hardcover ISBN: 978-3-662-50430-7Published: 28 June 2016
Softcover ISBN: 978-3-662-57058-6Published: 07 June 2018
eBook ISBN: 978-3-662-50432-1Published: 16 June 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 199
Topics: Microbiology, Biochemical Engineering