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Hydrocarbon and Lipid Microbiology Protocols

Biochemical Methods

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Hermann J. Heipieper
    Pages 1-2
  3. Thomas J. Smyth, Michelle Rudden, Konstantina Tsaousi, Roger Marchant, Ibrahim M. Banat
    Pages 3-28
  4. Thomas J. P. Smyth, Michelle Rudden, Konstantina Tsaousi, Roger Marchant, Ibrahim M. Banat
    Pages 29-60
  5. Tony Gutierrez, Ibrahim M. Banat
    Pages 61-74
  6. Forough Ghasemi Naghdi, Peer M. Schenk
    Pages 75-79
  7. Charlotte Wermser, Daniel Lopez
    Pages 113-122
  8. Nancy Hachicho, Hermann J. Heipieper
    Pages 123-132
  9. Rachel Narehood Austin, David Born, Thomas J. Lawton, Grace E. Hamilton
    Pages 133-147
  10. Tino Krell, Miriam Rico-Jiménez, Andrés Corral Lugo, José Antonio Reyes Darias, Álvaro Ortega, Abdelali Daddaoua
    Pages 177-188

About this book

Introduction

This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented.

Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Keywords

Biomarkers Hydrocarbon Biodegradation Hydrocarbon Transport Proteins Lipid Analysis Lipopeptide Analysis Protein Isolation

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of EssexColchester, EssexUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Dept. BiologyUniversity of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)"Palma de MallorcaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-49137-9
  • Copyright Information Springer-Verlag Berlin Heidelberg 2016
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-662-49135-5
  • Online ISBN 978-3-662-49137-9
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site