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Engineering of Halogenases towards Synthetic Applications

Increasing the Thermostability and Investigations on a Marine Brominase Bmp5

  • Hannah Minges

Part of the BestMasters book series (BEST)

Table of contents

  1. Front Matter
    Pages I-XII
  2. Hannah Minges
    Pages 13-24
  3. Hannah Minges
    Pages 25-25
  4. Hannah Minges
    Pages 27-57
  5. Hannah Minges
    Pages 59-79
  6. Hannah Minges
    Pages 81-87
  7. Hannah Minges
    Pages 109-119
  8. Hannah Minges
    Pages 121-122
  9. Hannah Minges
    Pages 123-126
  10. Back Matter
    Pages 127-128

About this book

Introduction

Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place. 

Contents
  • Halogenating Enzymes
  • Identification of a Thermostable Thal Variant by Directed Evolution
  • Establishment of the Brominase Bmp5 for Synthetic Application 
Target Groups
Lecturers, students and researchers in biological chemistry and chemistry

The Author
Hannah Minges, a Ph.D student at Bielefeld University, works on the optimization of tryptophan halogenases by means of directed evolution.

Keywords

Thal Bmp5 Directed Evolution Enzyme Biocatalysis

Authors and affiliations

  • Hannah Minges
    • 1
  1. 1.BielefeldGermany

Bibliographic information