Plant Cell and Tissue Culture

A Laboratory Manual

  • Jakob Reinert
  • Michael Magson Yeoman

Table of contents

  1. Front Matter
    Pages i-v
  2. Introduction

    1. Jakob Reinert, Michael Magson Yeoman
      Pages 1-1
  3. Isolation of Plant Material and Studies on Growth and Cell Division

    1. Front Matter
      Pages 3-3
    2. Jakob Reinert, Michael Magson Yeoman
      Pages 8-10
  4. Bioassay Systems for Cytokinins

    1. Front Matter
      Pages 19-19
    2. Jakob Reinert, Michael Magson Yeoman
      Pages 20-21
    3. Jakob Reinert, Michael Magson Yeoman
      Pages 22-23
  5. Morphogenesis in Vitro: Studies on Regeneration

    1. Front Matter
      Pages 25-25
    2. Jakob Reinert, Michael Magson Yeoman
      Pages 26-28
    3. Jakob Reinert, Michael Magson Yeoman
      Pages 29-31
    4. Jakob Reinert, Michael Magson Yeoman
      Pages 35-38
  6. Isolation, Culture and Fusion of Protoplasts from Higher Plants

    1. Front Matter
      Pages 39-39
    2. Jakob Reinert, Michael Magson Yeoman
      Pages 40-43
    3. Jakob Reinert, Michael Magson Yeoman
      Pages 44-45
  7. Secondary Metabolites in Tissue Cultures

    1. Front Matter
      Pages 47-47

About this book

Introduction

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu­ are now established in many research laboratories racy with sufficient illustration to make all mani­ throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec­ and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Keywords

Cell Gewebskultur Tissue Viruses Zellkultur

Authors and affiliations

  • Jakob Reinert
    • 1
  • Michael Magson Yeoman
    • 2
  1. 1.Institut für Pflanzenphysiologie und ZellbiologieBerlin 33Federal Republic of Germany
  2. 2.Department of BotanyUniversity of EdinburghEdinburghGreat Britain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-81784-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 1982
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-81786-1
  • Online ISBN 978-3-642-81784-7
  • About this book