Pollen Biology

A Laboratory Manual

  • K. R. Shivanna
  • N. S. Rangaswamy

Table of contents

  1. Front Matter
    Pages I-XI
  2. K. R. Shivanna, N. S. Rangaswamy
    Pages 1-3
  3. K. R. Shivanna, N. S. Rangaswamy
    Pages 5-7
  4. K. R. Shivanna, N. S. Rangaswamy
    Pages 9-22
  5. K. R. Shivanna, N. S. Rangaswamy
    Pages 23-31
  6. K. R. Shivanna, N. S. Rangaswamy
    Pages 33-37
  7. K. R. Shivanna, N. S. Rangaswamy
    Pages 39-43
  8. K. R. Shivanna, N. S. Rangaswamy
    Pages 45-60
  9. K. R. Shivanna, N. S. Rangaswamy
    Pages 61-72
  10. K. R. Shivanna, N. S. Rangaswamy
    Pages 73-83
  11. K. R. Shivanna, N. S. Rangaswamy
    Pages 85-102
  12. Back Matter
    Pages 103-119

About this book

Introduction

Pollen grains are everywhere - in the air, in the water, in soil and in the food we eat. Pollen has direct relevance in agriculture, horticulture, plant breeding, crop improvement and biotechnology. They are further of use for montioring cytotoxic effects - by herbicides, pesticides and pollutants - testing for allergic reactions, and for basic studies on gene expression, research on differentiation and polarity. Detailed instructions of the standard techniques, which have all been tested and improvised by the authors, are given, such as collection and storage of pollen, pollen culture, germination, tests for viability, incompatibility and isolation of protoplasts. Introduced by an explanation of the principles involved, the step-by step protocols are complemented by personal notes and precautions, specifying the reagents used and various appendices on basic and specific requirements for laboratory exercises on pollen.

Keywords

Embryo Keimen Pflanzenkulturen Pflanzenreproduktion Pollen Protoplasten Translation biotechnology cell culture plant reproduction protoplasts germination temperature transcription

Authors and affiliations

  • K. R. Shivanna
    • 1
  • N. S. Rangaswamy
    • 1
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-77306-8
  • Copyright Information Springer-Verlag Berlin Heidelberg 1992
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-55170-6
  • Online ISBN 978-3-642-77306-8
  • About this book