Fine Structure Immunocytochemistry

  • Gareth Griffiths

Table of contents

  1. Front Matter
    Pages I-XXI
  2. Gareth Griffiths
    Pages 9-25
  3. Gareth Griffiths
    Pages 90-136
  4. Gareth Griffiths
    Pages 137-203
  5. Brian Burke
    Pages 204-236
  6. Gareth Griffiths
    Pages 237-278
  7. Gareth Griffiths
    Pages 345-370
  8. Gareth Griffiths
    Pages 371-445
  9. Back Matter
    Pages 451-459

About this book


Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back­ ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi­ quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess­ arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this


antibody biology chemistry electron microscopy enzyme enzymes ferritin glycoprotein membrane microscopy molecular biology protein proteins receptor tissue

Authors and affiliations

  • Gareth Griffiths
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1993
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-77097-5
  • Online ISBN 978-3-642-77095-1
  • Buy this book on publisher's site