PCR Topics

Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

  • Arndt Rolfs
  • Hermann Christian Schumacher
  • Peter Marx

Table of contents

  1. Front Matter
    Pages I-XI
  2. Basic Methodology and Research Applications

    1. Front Matter
      Pages 1-1
    2. Alice M. Wang, Michael V. Doyle, David F. Mark
      Pages 3-8
    3. Hartmut Hampl, Ronald A. Marshall, Timothy Perko, Natalie Solomon
      Pages 15-22
    4. Lutz-Peter Berg, David S. Millar, Catherine B. Grundy, Kerstin Wieland, Jonathan K. Pattinson, Edward G. D. Tuddenham et al.
      Pages 23-31
    5. Jörg Ehrenpreis, Barbara Junkes, Martin Pfordt, Hans-Peter Vosberg
      Pages 32-40
    6. G. Röllich, M. Jafari
      Pages 46-47
    7. M. E. Westarp, B. Föhring
      Pages 48-52
    8. Rüdiger Rüger, Hans-Joachim Höltke, Udo Reischl, Gregor Sagner, Christoph Kessler
      Pages 56-58
    9. Oliva Handt, André Reis, Rudolf Häring, Jörg Schmidtke
      Pages 63-65
    10. Erik C. Böttger
      Pages 66-68
    11. Armin Wagner, Bernd Reckmann, Kerstin Hagen-Mann, Gerhard Krauss
      Pages 69-71
  3. Genetics and Evolution

About this book

Introduction

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Keywords

DNA detection methods Polymerase chain reaction (PCR) Viral diseases AIDS antigen bacteria diseases genetics Hepatitis infection infections infectious infectious disease infectious diseases lymphokine retroviruses tuberculosis virus

Editors and affiliations

  • Arndt Rolfs
    • 1
  • Hermann Christian Schumacher
    • 1
  • Peter Marx
    • 1
  1. 1.Neurologische Klinik des Klinikum SteglitzFU BerlinBerlin 45Germany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-75924-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 1991
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-52934-7
  • Online ISBN 978-3-642-75924-6
  • About this book