Overview
- Editors:
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Y. P. S. Bajaj
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New Delhi, India
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Table of contents (28 chapters)
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Isolation, Culture, and Preservation of Protoplasts
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- J. J. M. Dons, C. M. Colijn-Hooymans
Pages 50-62
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- H. Schnabl, U. Zimmermann
Pages 63-96
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Regeneration of Plants from Protoplasts
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Front Matter
Pages 107-107
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- J. Kyozuka, K. Shimamoto, H. Ogura
Pages 109-123
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- M. N. Barakat, E. C. Cocking
Pages 160-172
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- Y. Yamashita, K. Shimamoto
Pages 193-205
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- Kazunori Yabe, Takeshi Nishio, Kenji Takayanagi
Pages 227-234
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- Charlene J. Boyes, Kenneth C. Sink
Pages 254-261
About this book
Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-ยท toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean preparaยญ tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Interยญ national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, inยญ corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.
Editors and Affiliations
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New Delhi, India
Y. P. S. Bajaj