Hormonally Defined Media

1. Front Matter

   Pages I-XIV

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2. Opening Lecture

   1. Front Matter

      Pages 1-1

      PDF

   2. Divining the Role of Serum in Cell Culture

      • Gordon H. Sato

      Pages 2-3

3. Nutritional Requirements

   1. Front Matter

      Pages 5-5

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   2. Reducing the Requirement for Serum Supplements in High Yield Microcarrier Cell Culture

      • Julian Clark

      Pages 6-15

   3. Growth of Normal Human Cells in Defined Media

      • Richard G. Ham

      Pages 16-30

   4. Investigations on the Cultivation of Insect Cell Lines in Serum-Free Media

      • H. G. Miltenburger

      Pages 31-43

4. Cell Proliferation and Growth Factors

   1. Front Matter

      Pages 45-45

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   2. Different Signalling Systems Control the Initiation of DNA Replication in Cultured Animal Cells

      • Luis Jimenez de Asua, Angela M. Otto

      Pages 46-56

   3. Ectopic Auto-Stimulation of Growth

      • Robert R. Bürk

      Pages 57-67

   4. The Growth and Differentiation of Human Endothelial Cells

      • T. Maciag, R. Weinstein

      Pages 68-78

   5. 3T3 Cell Growth Initiation and Multiplication in Defined Medium Supplemented with Growth Factors and Hormones That Replace Serum

      • Dieter Paul

      Pages 79-87

   6. Growth-Promoting Activity of Thrombin in Fibroblasts: Temporal Action and Early Biochemical Events in the G0 → G1 Transition

      • J. Pouysségur, J. C. Chambard, A. Franchi, G. L’Allemain, S. Paris, E. Van Obberghen-Schilling

      Pages 88-102

   7. Pure Platelet-Derived Growth Factor Stimulates Human Fibroblasts to Proliferate in Plasma-Free Culture

      • C. Betsholtz, C.-H. Heldin, Å. Wasteson, B. Westermark

      Pages 103-106

   8. Synergistic Effect of Retinoic Acid on DNA Synthesis of Prostaglandin F2α Stimulated Swiss 3T3 Cells

      • M. Eschenbruch, A. M. Otto, L. Jimenez de Asua

      Pages 107-110

   9. The Secretion of Growth Regulatory Molecules by PC13 Embryonal Carcinoma Cells

      • John K. Heath, Clare M. Isacke

      Pages 111-113

   10. Growth Regulation of L-929 Mouse Fibroblasts by Steroid Hormones and Anti-Hormones in Serum Containing and Serum Free Media

       • I. Jung-Testas, E. E. Baulieu

       Pages 114-119

   11. Synergistic Interactions of Specific Prostaglandins in Regulating the Rate of Initiation of DNA Synthesis in Swiss 3T3 Cells

       • Angela M. Otto, Marit Nilsen-Hamilton, Barbara Boss, Luis Jimenez de Asua

       Pages 120-122

   12. Use of Eye Derived Growth Factor from Retina (EDGF) in a Defined Medium for the Culture of Bovine Epithelial Lens Cells

       • J. Plouët, M. Olivié, J. Courty, Y. Courtois, D. Barritault

       Pages 123-126

   13. Effect of Partially Purified Preparation of Human Somatomedin A in the Three Cultured Cell Systems

       • J. Straczek, M. H. Heulin, F. Sarem, A. Lasbennes, M. Artur, C. Geschier et al.

       Pages 127-130

Until some years ago serum or crude tissue extracts were used pre­ dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup­ plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer­ ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re­ gard to survival, proliferation and differentiation of cells in cul­ ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti­ vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.

• Fischer
• Hormonhaltiger serumfreier Nährboden
• Zellkultur
• biology
• cell
• cell biology
• cell culture
• influence
• tissue

• Institut für Neurobiologie, Universität Heidelberg, Heidelberg 1, Germany

  G. Fischer

• Institut für Pharmakologie, Universität Mainz, Mainz, Germany

  R. J. Wieser

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