Advertisement

Diffusion Chamber Culture

Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays

  • Eugene P. Cronkite
  • Arland L. Carsten
Conference proceedings

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Characterization of Progenitor Cells in Diffusion Chambers

  3. Regulatory Mechanisms in Diffusion Chamber Cell Growth

  4. Cell Interactions, Cytogenetics, and Methodology

  5. Characterization of Tumour and Leukemic Cell Growth

    1. Front Matter
      Pages 213-213
    2. S. Öhl, S. Seeber, W. R. Boecker, C. G. Schmidt
      Pages 215-223
    3. W. R. Boecker, D. K. Hossfeld, S. Öhl, C. G. Schmidt, O. Wetter
      Pages 224-233
    4. D. Hoelzer, E. B. Harriss, B. Bültmann, T. M. Fliedner, H. Heimpel
      Pages 242-250
    5. E. S. Handler, B. Semel, H. N. Steinberg
      Pages 251-258
  6. Erythropoiesis in Plasma Clot Diffusion Chamber (PCDC) Culture

  7. Summary and Conclusions

    1. E. P. Cronkite
      Pages 263-268
  8. Back Matter
    Pages 269-277

About these proceedings

Introduction

Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question.

Keywords

Diffusion cell cytogenetics genetics leukemia mutagen tumor

Editors and affiliations

  • Eugene P. Cronkite
    • 1
  • Arland L. Carsten
    • 1
  1. 1.Medical DepartmentBrookhaven National LaboratoryUptonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-67644-4
  • Copyright Information Springer-Verlag Berlin Heidelberg 1980
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-10064-5
  • Online ISBN 978-3-642-67644-4
  • Buy this book on publisher's site