Red Cell Rheology

  • Marcel Bessis
  • Stephen B. Shohet
  • N. Mohandas

Table of contents

  1. Front Matter
    Pages 1-7
  2. Part I

    1. Front Matter
      Pages 9-9
    2. Methods for Evaluation of Red Cell Deformability

    3. Biochemical Basis for Red Cell Shape and Deformability

      1. K. Tadano, J. D. Hellums, E. C. Lynch, E. J. Peck, C. P. Alfrey
        Pages 163-174
      2. Robert S. Heusinkveld, David A. Goldstein, Robert I. Weed, Paul L. Lacelle
        Pages 175-182
      3. Robert S. Heusinkveld, David A. Goldstein, Robert I. Weed, Paul Lacelle
        Pages 183-184
    4. Clinical Applications

  3. Part II

    1. Front Matter
      Pages 223-223
    2. Sickle Cell Rheology

      1. Marcel Bessis, Narla Mohandas
        Pages 225-235

About these proceedings

Introduction

Hemolysis during filtration through micropores studied by Chien et al. [I] showed a dependence on pressure gradient and pore diameter that, at the time of publication, did not permit an easy interpretation of the hemolytic mechanism. Acting on the assumption that thresholds of hemolysis are easier to correlate with physical forces than extents of hemolysis, we performed a series of experi­ ments repeating some of the conditions reported in [I] and then focusing on low L1P in order to define better the thresholds of hemolysis for several pore sizes. Employing a model of a deformed red cell shape at the pore entrance (based on micropipette observations) we related the force field in the fluid to a biaxial tension in the membrane. The threshold for lysis correlated with a membrane tension of 30 dynes/cm. This quantity is in agreement with lysis data from a number of other investigators employing a variety of mechanisms for introduc­ ing membrane tension. The sequence of events represented here is: a. Fluid forces and pressure gradients deform the cell into a new, elongated shape. b. Extent of deformation becomes limited by the resistance of the cell mem­ brane to undergo an increase in area. c. Fluid forces and pressure gradients acting on the deformed cell membrane cause an increase in biaxial tension in the membrane. d. When the strain caused by this tension causes pores to open in the membrane, the threshold for hemolysis has been reached [2].

Keywords

Rheologie Rheology Rotes Blutkörperchen Sichelzellanämie behavior cell cell membrane membrane protein

Editors and affiliations

  • Marcel Bessis
    • 1
  • Stephen B. Shohet
    • 2
  • N. Mohandas
    • 3
  1. 1.Institut de Pathologie CellulaireHôpital de BicêtreBicêtreFrance
  2. 2.School of Medicine, Cancer Research CenterThe University of CaliforniaSan FranciscoUSA
  3. 3.School of Medicine, Cancer Research InstituteUniversity of CaliforniaSan FranciscoUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-67059-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 1978
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-09001-4
  • Online ISBN 978-3-642-67059-6
  • About this book