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Freeze-Etch Histology

A Comparison between Thin Sections and Freeze-Etch Replicas

  • Lelio Orci
  • Alain Perrelet

Table of contents

  1. Front Matter
    Pages I-IX
  2. Lelio Orci, Alain Perrelet
    Pages 1-2
  3. Lelio Orci, Alain Perrelet
    Pages 3-9
  4. Lelio Orci, Alain Perrelet
    Pages 10-49
  5. Lelio Orci, Alain Perrelet
    Pages 51-57
  6. Lelio Orci, Alain Perrelet
    Pages 59-73
  7. Lelio Orci, Alain Perrelet
    Pages 75-81
  8. Lelio Orci, Alain Perrelet
    Pages 83-97
  9. Lelio Orci, Alain Perrelet
    Pages 99-105
  10. Lelio Orci, Alain Perrelet
    Pages 107-121
  11. Lelio Orci, Alain Perrelet
    Pages 123-129
  12. Lelio Orci, Alain Perrelet
    Pages 131-133
  13. Lelio Orci, Alain Perrelet
    Pages 135-149
  14. Lelio Orci, Alain Perrelet
    Pages 151-157
  15. Lelio Orci, Alain Perrelet
    Pages 159-161
  16. Lelio Orci, Alain Perrelet
    Pages 163-165
  17. Back Matter
    Pages 167-170

About this book

Introduction

The development of the electron microscope brought one to the other. The purpose of this book is to in a new era, in which cell structures could be provide a bridge, to help those who are familiar visualized down to a macromolecular level. It is of with conventional electron microscopy, to cross course well recognized that some cell components into the less familiar freeze-etching. This has been can be modified or even completely lost during the done by providing paired photographs wherever complex sequence of fixation, dehydration, staining possible, one showing the thin section of a tissue or and plastic embedding which is essential before a a cell, the other a freeze-etch replica of a similar thin section can be obtained. Further, only these cell material. At the same time we have tried to provide components which can be made electron opaque a basis for interpretation of freeze-etched structures, can be visualized. For these reasons, the morpho­ and we hope the reader will find the first 23 Plates logist and cell biologist alike have to explore alter­ especially useful in this respect. Because there are native techniques of specimen preparation which many excellent atlases of cell structure available at may avoid some of the artefacts inherent in con­ the present time, we felt there was no need to ventional thin-section electron microscopy, and at provide lengthy discussions of each plate.

Keywords

Gefrierätzung Zelle cell electron microscopy histology tissue

Authors and affiliations

  • Lelio Orci
    • 1
  • Alain Perrelet
    • 1
  1. 1.Institute of Histology and Embryology, School of MedicineUniversity of GenevaGenevaSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-66020-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 1975
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-66022-1
  • Online ISBN 978-3-642-66020-7
  • Buy this book on publisher's site