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  • Book
  • © 2002

FISH Technology

  • Over 40 brillant colour photos showing the results of fluorescence in situ hybridization on various tissues

  • Comprehensive collection of proven lab protocols, each provided by an expert in the field

  • Detailed step-by-step protocols, including troubleshooting sections and practical comments

  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-642-56404-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (36 chapters)

  1. Front Matter

    Pages I-X
  2. The Standard FISH Procedure — an Introduction

    1. Front Matter

      Pages 1-1
    2. Microscopy and Imaging Systems

      • Andreas Plesch
      Pages 51-69
  3. FISH on Native, Human Tissues

    1. Front Matter

      Pages 71-71
    2. FISH on Chromosome Preparations of Peripheral Blood

      • Thomas Liehr, Uwe Claussen
      Pages 73-81
    3. FISH on Uncultured Blood or Bone Marrow

      • Thomas Liehr
      Pages 82-89
    4. FISH on Blood or Bone Marrow Smears

      • Thomas Liehr
      Pages 90-96
    5. FISH on Hair Root Cells

      • Thomas Liehr
      Pages 109-114
    6. Amniotic Fluid Cells — Uncultured

      • Renate Ulmer
      Pages 115-126
    7. Human Sperm Cells

      • Anita Rauch
      Pages 127-137
  4. FISH on Archival, Human Tissues

    1. Front Matter

      Pages 139-139
    2. Archival Cytogenetic Slides

      • Erich Gebhart
      Pages 141-147
    3. Formalin-Fixed and Paraffin-Embedded Tissue Sections

      • Harald Rieder, Christine Sommerlad, Yasmin Mehraein, Marina Giersberg, Kersten Marben, Helga Rehder
      Pages 148-161
    4. Nucleus Extraction from Cryofixed Tissue

      • Bernd Rautenstrauss, Thomas Liehr
      Pages 176-184
    5. Comparative Genomic Hybridization

      • Burt G. Feuerstein, Yuichi Hirose, Malgorzata Pellarin, Gregg Magrane
      Pages 197-217

Keywords

  • Chromosom
  • DNA
  • FISH
  • Fluoreszenz in situ-Hybridisierung
  • Interphase
  • PCR
  • Telomere
  • chromosome
  • genetics
  • genomic alterations
  • hybridization
  • in situ hybridization
  • microscopy
  • tissue
  • translation

Reviews

From the reviews:

"Ever wondered how to catch your FISH … in the dark pools of cellular biology? Then … FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) … . Every chapter is illustrated by several schematic drawings … . The manual is completed by a subject index … . The book is certainly clear … providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

Editors and Affiliations

  • Institut für Humangenetik, Universität Erlangen-Nürnberg, Erlangen, Germany

    Bernd W. Rautenstrauss

  • Institut für Humangenetik, Universität Jena, Jena, Germany

    Thomas Liehr

Bibliographic Information

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-642-56404-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)