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  • © 2014

Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

Authors:

  • Nominated by Chinese Academy of Sciences as an outstanding PhD thesis

  • Describes the first example of online multidimensional chromatography separation using capillary monolithic columns

  • Introduces the combination of online stable isotope labeling and multidimensional chromatography separation

  • Outlines the proteome and phosphoproteome expression profiles of clinical hepatocellular carcinoma tissues and hippocampi of mice with Alzheimer’s disease

  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Theses (Springer Theses)

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  • ISBN: 978-3-642-42008-5
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About this book

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity, and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

Keywords

  • Isotope Dimethylation Labeling
  • Mass Spectrometry
  • Multidimensional Chromatography Separation
  • Polymer-Based Hydrophobic Monolithic Column
  • Protein Phosphorylation
  • Proteome Analysis
  • Quantitative Proteomics
  • Separation of Complex Peptide Mixtures
  • Stable Isotope Labeling
  • Strong Cation-Exchange Monolithic Column
  • chromatography
  • Proteomics

Authors and Affiliations

  • Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, People's Republic of China

    Fangjun Wang

Bibliographic Information

  • Book Title: Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

  • Authors: Fangjun Wang

  • Series Title: Springer Theses

  • DOI: https://doi.org/10.1007/978-3-642-42008-5

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2014

  • Hardcover ISBN: 978-3-642-42007-8

  • Softcover ISBN: 978-3-662-52432-9

  • eBook ISBN: 978-3-642-42008-5

  • Series ISSN: 2190-5053

  • Series E-ISSN: 2190-5061

  • Edition Number: 1

  • Number of Pages: XIII, 94

  • Number of Illustrations: 19 b/w illustrations, 23 illustrations in colour

  • Topics: Separation Science, Protein Biochemistry, Mass Spectrometry

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • ISBN: 978-3-642-42008-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 159.99
Price excludes VAT (USA)