Skip to main content
  • Book
  • © 2012

Culture Negative Orthopedic Biofilm Infections

  • Introduces the best diagnostic technologies that modern microbiology can provide

  • Two of the editors (Ehrlich and Costerton) are involved in the development of an automated DNA-based system for the identification of bacteria and fungi in clinical specimens, by Abbott Healthcare

  • Constitutes a cogent statement of the reasons for the replacement of traditional culture methods, with DNA-based systems, in all medical specialities

  • Definitive source of the concepts and the preliminary data on which this huge paradigm shift will be based

  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Series on Biofilms (BIOFILMS, volume 7)

Buy it now

Buying options

Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Table of contents (10 chapters)

  1. Front Matter

    Pages i-x
  2. The Problem of Culture-Negative Infections

    • G. D. Ehrlich, P. J. DeMeo, J. W. Costerton
    Pages 1-15
  3. Culture-Negative Infections in Orthopedic Surgery

    • G. D. Ehrlich, Patrick DeMeo, Michael Palmer, T. J. Sauber, Dan Altman, Greg Altman et al.
    Pages 17-27
  4. Improved Diagnosis of Biofilm Infections Using Various Molecular Methods

    • Trine Rolighed Thomsen, Yijuan Xu, Jan Lorenzen, Per Halkjær Nielsen, Henrik Carl Schønheyder
    Pages 29-41
  5. Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention

    • Nathan K. Archer, J. William Costerton, Jeff G. Leid, Mark E. Shirtliff
    Pages 61-75
  6. Towards a New Paradigm in the Diagnosis and Treatment of Orthopedic Infections

    • G. D. Ehrlich, J. W. Costerton, D. Altman, G. Altman, M. Palmer, C. Post et al.
    Pages 129-139
  7. Back Matter

    Pages 141-144

About this book

During the recent transition between acute diseases caused by swarms of single planktonic bacteria, and chronic infections caused by bacteria growing in slime-enclosed biofilms, a general clinical consensus has emerged that pathologies with bacterial etiologies are frequently culture negative. Because biofilm infections now affect 17 million Americans per year (killing approximately 450,000), the suggestion that these common and lethal infections regularly go unnoticed by the only FDA-approved method for their detection and characterization is a matter of urgent concern. Biologically, we would expect that planktonic bacterial cells would colonize any new surface, including the surface of an agar plate, while the specialized sessile cells of a biofilm community would have no such proclivity. In the study of biofilm diseases ranging from otitis media to prostatitis, it was found that direct microscopy and DNA- and RNA-based molecular methods regularly document the presence of living bacteria in tissues and samples that are culture negative. The editors selected orthopedic biofilm infections as the subject of this book because these infections occur against a background of microbiological sterility in which modern molecular methods would be expected to find bacterial DNA, RNA-based microscopic methods would be expected to locate bacterial cells, and cultures would be negative. Moreover, in Orthopedics we find an already biofilm-adapted surgical group in which current strategies are based on the meticulous removal of compromised tissues, antibiotic options as based on high biofilm-killing local doses, and there are practical bedside strategies for dealing with biofilm infections. So here is where the new paradigm of biofilm infection meets the equally new paradigm of the culture negativity of biofilms, and this volume presents a conceptual synthesis that may soon combine the most effective molecular methods for the detection and identification of bacteria with a surgical discipline that is ready to help patients.

Editors and Affiliations

  • Center for Genomic Sciences, Allegheny-Singer Research Inst., Pittsburgh, USA

    Garth D. Ehrlich

  • , Department of Orthopedic Surgery, Allegheny General Hospital, Pittsburgh, USA

    Patrick J. DeMeo

  • Center for Genomic Sciences, Biofilm Research, Allegheny-Singer Research Institute, Pittsburgh, USA

    J. William Costerton

  • Privatklinik Döbling GmbH, Wien, Austria

    Heinz Winkler

Bibliographic Information

  • Book Title: Culture Negative Orthopedic Biofilm Infections

  • Editors: Garth D. Ehrlich, Patrick J. DeMeo, J. William Costerton, Heinz Winkler

  • Series Title: Springer Series on Biofilms

  • DOI: https://doi.org/10.1007/978-3-642-29554-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2012

  • Hardcover ISBN: 978-3-642-29553-9Published: 18 December 2012

  • Softcover ISBN: 978-3-642-44683-2Published: 29 January 2015

  • eBook ISBN: 978-3-642-29554-6Published: 18 December 2012

  • Series ISSN: 1863-9607

  • Series E-ISSN: 1863-9615

  • Edition Number: 1

  • Number of Pages: X, 146

  • Topics: Microbiology, Bacteriology, Microbial Ecology

Buy it now

Buying options

Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access