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Guidelines for Molecular Analysis in Archive Tissues

  • Giorgio Stanta

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Archive Tissues

    1. Front Matter
      Pages 1-1
    2. Giorgio Stanta
      Pages 3-4
    3. Lorenzo Daniele, Giuseppe D’Armento, Gianni Bussolati
      Pages 5-11
    4. Isabella Dotti, Serena Bonin, Giorgio Basili, Valentina Faoro
      Pages 13-18
  3. Dissection of Tissue Components

    1. Front Matter
      Pages 19-19
    2. Valentina Faoro, Giorgio Stanta
      Pages 21-22
    3. Valentina Faoro, Anna Sapino
      Pages 23-26
    4. Elvira Stacher, Hannelore Kothmaier, Iris Halbwedl, Helmut H. Popper
      Pages 27-29
  4. Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids

    1. Front Matter
      Pages 31-31
    2. Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper
      Pages 33-36
    3. Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper
      Pages 37-39
    4. Solange Sorçaburu Cigliero, Elisabetta Edalucci, Paolo Fattorini
      Pages 45-54
    5. Serena Bonin, Federica Tavano
      Pages 55-56
    6. Serena Bonin, Giorgio Stanta
      Pages 57-61
    7. Marco Alberghini, Stefania Benini, Gabriella Gamberi, Stefania Cocchi, Licciana Zanella
      Pages 63-66
    8. Serena Bonin, Giorgio Stanta
      Pages 67-69
    9. Roberto Cirombella, Andrea Vecchione
      Pages 71-74
    10. Isabella Dotti, Serena Bonin
      Pages 75-79
    11. Isabella Dotti, Serena Bonin
      Pages 81-85
    12. Isabella Dotti, Serena Bonin
      Pages 87-90
  5. Reverse Transcription (RT)

    1. Front Matter
      Pages 91-91
    2. Isabella Dotti, Serena Bonin, Ermanno Nardon
      Pages 93-97
  6. Nucleic Acid Amplification

    1. Front Matter
      Pages 99-99
    2. Serena Bonin, Isabella Dotti
      Pages 101-103
    3. Serena Bonin, Isabella Dotti
      Pages 105-109
    4. Serena Bonin, Isabella Dotti
      Pages 111-114
    5. Serena Bonin, Isabella Dotti
      Pages 115-116
    6. Christiane Schewe, Wilko Weichert, Manfred Dietel
      Pages 117-120
    7. Isabella Dotti, Ermanno Nardon, Danae Pracella, Serena Bonin
      Pages 121-132
  7. DNA Sequencing

    1. Front Matter
      Pages 133-133
    2. Giorgio Basili, Serena Bonin, Isabella Dotti
      Pages 135-141
    3. Gerlinde Winter, Gerald Höfler
      Pages 143-152
  8. Microsatellite Analysis

    1. Front Matter
      Pages 153-153
    2. Damjan Glavač, Ermanno Nardon
      Pages 155-169
    3. Damjan Glavač, Ermanno Nardon
      Pages 171-177
  9. Methylation Analysis

  10. Analysis of Copy Number Variations

    1. Front Matter
      Pages 201-201
    2. Hannelore Kothmaier, Elvira Stacher, Iris Halbwedl, Helmut H. Popper
      Pages 203-214
    3. Ana Pilar Berbegall, Eva Villamón, Samuel Navarro, Rosa Noguera
      Pages 215-224
    4. Marta Piqueras, Manish Mani Subramaniam, Samuel Navarro, Nina Gale, Rosa Noguera
      Pages 225-230
  11. Microarrays

    1. Front Matter
      Pages 231-231
    2. Manfred Dietel, Jan Budczies, Wilko Weichert, Carsten Denkert
      Pages 233-237
  12. Protein Extraction in Archive Tissues

    1. Front Matter
      Pages 239-239
    2. Valentina Faoro, Karl-Friedrich Becker, Giorgio Stanta
      Pages 241-243
    3. Karl-Friedrich Becker, Christina Schott
      Pages 245-248

About this book

Introduction

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.

 

This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Keywords

Fixed and Paraffin-embedded Tissues Molecular Diagnostics Translational Research

Editors and affiliations

  • Giorgio Stanta
    • 1
  1. 1.c/o Ospedale di CattinaraUniversity of TriesteTriesteItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-17890-0
  • Copyright Information Springer-Verlag Berlin Heidelberg 2011
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Medicine
  • Print ISBN 978-3-642-17889-4
  • Online ISBN 978-3-642-17890-0
  • Buy this book on publisher's site