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  • © 2009

Fluorescence In Situ Hybridization (FISH) - Application Guide


  • Step-by-step protocols of the cutting edge FISH techniques

  • Easy to follow recipies to ensure successful results

  • Each protocol includes interpretation and evaluation sections of the experimental results as well as troubleshooting tips

  • With brillant colour photos showing the results of fluorescence in situ hybridization on various tissues

  • Includes supplementary material:

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-3-540-70581-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (36 protocols)

  1. Front Matter

    Pages i-xviii
  2. Molecular Cytogenetics, Probes and Equipment

    1. Molecular Cytogenetics: The Standard FISH and PRINS Procedure

      • Thomas Liehr, Franck Pellestor
      Pages 23-34
    2. Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes

      • Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, Nigel P. Carter
      Pages 35-52
    3. ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results

      • Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka et al.
      Pages 53-60
    4. PNA-FISH Technique for In Situ Assessment of Aneuploidy

      • Franck Pellestor, Petra Paulasova, Samir Hamamah
      Pages 61-66
    5. Oligonucleotide FISH Probes

      • Sean Bradley, Leah Zamechek, Joan Aurich-Costa
      Pages 67-73
    6. Microscopy and Imaging Systems

      • Ivan Y. Iourov
      Pages 75-84
    7. Optical Filters for FISH

      • Michael Sommerauer, Ingrid Feuerbacher
      Pages 85-97
    8. FISH With and Without COT1 DNA

      • Vladimir A. Trifonov, Nadezhda N. Vorobieva, Willem Rens
      Pages 99-109
  3. FISH Applications in Different Tissue Preparations

    1. Prenatal Diagnostics on Uncultured Amniocytes

      • Bernd Eiben, Ralf Glaubitz, Arens Hans-Jürgen
      Pages 123-130
    2. Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears

      • Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan et al.
      Pages 131-136
    3. Application of FISH to Archival Cytogenetic Slides

      • Thomas Liehr, Erich Gebhart
      Pages 137-140
    4. Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine

      • Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, Thomas Liehr
      Pages 141-146
    5. FISH on Sperms, Spermatocytes and Oocytes

      • Maria Oliver-Bonet
      Pages 157-172
  4. Characterization of Marker Chromosomes

    1. Multiplex FISH and Spectral Karyotyping

      • Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko et al.
      Pages 175-181
    2. Tips and Tricks for mFISH

      • Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, Roderick A. F. MacLeod
      Pages 183-192
    3. Simultaneous Fluorescence Immunostaining and FISH

      • Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye et al.
      Pages 193-216

About this book

This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given.

Described here are methods using various cell types and tissues as well as different organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced for single cell analysis in human genetics, microbiology, animal and plant sciences.


  • Chromosom
  • Comparative genomic hybridization
  • Cytogenetics
  • DNA
  • Epigenetic aberrations
  • Fluoreszenz in situ-Hybridisierung
  • Interphase
  • Nuclear architecture
  • Termination
  • bone
  • genetics
  • hybridization
  • microscopy
  • tissue


From the review of the previous edition "FISH Technology - Springer Lab Manual", edited by B. Rautenstrauß and T. Liehr

"It is a book that would complement the library of any lab performing FISH experiments and would bring new technologies to labs already scaling the heights or to labs setting out on their journey. … One of the strengths of this book is the description of FISH techniques that have been developed to answer more specific questions about DNA structure and behaviour. … My PhD student has recently been using this book and praised it without knowing I was reviewing it – what better endorsement could there be?" (Joanna M. Bridger, Cell and Chromosome Biology Group, Brunel University, Middlesex, UK, Journal of Cell Science 116, 2375-2376 © 2003 The Company of Biologists Ltd doi:10.1242/jcs.00594)

"Ever wondered how to catch your FISH … in the dark pools of cellular biology? Then … FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) … . Every chapter is illustrated by several schematic drawings … . The manual is completed by a subject index … . The book is certainly clear … providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

Editors and Affiliations

  • Institut für Humangenetik und Anthropologie, Jena, Germany

    Thomas Liehr

Bibliographic Information

  • Book Title: Fluorescence In Situ Hybridization (FISH) - Application Guide

  • Editors: Thomas Liehr

  • DOI:

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag GmbH Germany, part of Springer Nature 2009

  • Softcover ISBN: 978-3-642-08952-7

  • eBook ISBN: 978-3-540-70581-9

  • Edition Number: 1

  • Number of Pages: XVIII, 451

  • Topics: Cell Biology, Medical Genetics

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-3-540-70581-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)