Fluorescence In Situ Hybridization (FISH) — Application Guide

  • Thomas Liehr

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Molecular Cytogenetics, Probes and Equipment

    1. Thomas Liehr, Franck Pellestor
      Pages 23-34
    2. Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, Nigel P. Carter
      Pages 35-52
    3. Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka et al.
      Pages 53-60
    4. Franck Pellestor, Petra Paulasova, Samir Hamamah
      Pages 61-66
    5. Sean Bradley, Leah Zamechek, Joan Aurich-Costa
      Pages 67-73
    6. Ivan Y. Iourov
      Pages 75-84
    7. Michael Sommerauer, Ingrid Feuerbacher
      Pages 85-97
    8. Vladimir A. Trifonov, Nadezhda N. Vorobieva, Willem Rens
      Pages 99-109
  3. FISH Applications in Different Tissue Preparations

    1. Bernd Eiben, Ralf Glaubitz, Arens Hans-Jürgen
      Pages 123-130
    2. Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan et al.
      Pages 131-136
    3. Thomas Liehr, Erich Gebhart
      Pages 137-140
    4. Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, Thomas Liehr
      Pages 141-146
    5. Maria Oliver-Bonet
      Pages 157-172
  4. Characterization of Marker Chromosomes

    1. Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko et al.
      Pages 175-181
    2. Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, Roderick A. F. MacLeod
      Pages 183-192
    3. Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye et al.
      Pages 193-216
    4. Luitpold Distel, Ulrike Keller, Susann Neubauer
      Pages 231-241
    5. Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Heike Nelle, Vladimir Trifonov, Marina Manvelyan et al.
      Pages 243-250
    6. Nadezda Kosyakova, Anja Weise, Kristin Mrasek, Hasmik Mkrtchyan, Vivien Klaschka, Elisabeth Ewers et al.
      Pages 251-257
  5. Characterization of Cryptic and Epigenetic Aberrations

    1. Anja Weise, Kristin Mrasek, Madeleine Gross, Vivien Klaschka, Thomas Liehr
      Pages 261-268
    2. Lu Chun-Mei, Mei Wang, Karin M. Greulich-Bode, F. Weier Jingly, G. Weier Heinz-Ulli
      Pages 269-291
    3. Anja Weise, Kristin Mrasek, Mkrtchyan Hasmik, Madeleine Gross, Sophie Hinreiner, Witthuhn Vera et al.
      Pages 293-297
  6. Interphase FISH and Characterization of the Nuclear Architecture

    1. Ivan Y. Iourov, Svetlana G. Vorsanova, Ilia V. Soloviev, Yuri B. Yurov
      Pages 301-311
    2. Friederike Hunstig, Marina Manvelyan, Samarth Bhatt, Ulf Steinhaeuser, Thomas Liehr
      Pages 313-320
  7. Applications of FISH in Zoology, Botany and Microbiology

    1. Fengtang Yang, Alexander S. Graphodatsky
      Pages 323-346
    2. Peng Zhang, Bernd Friebe
      Pages 365-394
    3. Benedetta Bottari, Danilo Ercolini, Monica Gatti, Erasmo Neviani
      Pages 395-408
  8. Comparative Genomic Hybridization (CGH) and Array CGH

    1. Joris R. Vermeesch
      Pages 421-437
  9. Internet Links for FISH

    1. Thomas Liehr, Anja Weise
      Pages 441-445
  10. Back Matter
    Pages 447-451

About this book


This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given.

Described here are methods using various cell types and tissues as well as different organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced for single cell analysis in human genetics, microbiology, animal and plant sciences.


Chromosom Comparative genomic hybridization Cytogenetics DNA Epigenetic aberrations Fluoreszenz in situ-Hybridisierung Interphase Nuclear architecture PRINS Termination bone genetics hybridization microscopy tissue

Editors and affiliations

  • Thomas Liehr
    • 1
  1. 1.Institut für Humangenetik und AnthropologieJenaGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2009
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-540-70580-2
  • Online ISBN 978-3-540-70581-9
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site