Advanced Techniques in Diagnostic Microbiology

1. Front Matter

   Pages i-xvii

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2. Bacterial Identification Based on Universal Gene Amplification and Sequencing

   • Susanna K. P. Lau, Jade L. L. Teng, Patrick C. Y. Woo

   Pages 1-29

3. Molecular Techniques for Blood and Blood Product Screening

   • Yuan Hu

   Pages 31-66

4. Molecular Diagnostics of Sexually Transmitted Diseases: Bacterial, Trichomonas, and Herpes Simplex Virus Infections

   • Kenneth L. Muldrew

   Pages 67-100

5. Advances in the Diagnosis of Mycobacterium tuberculosis Infection

   • Duanne Alves da Silva, Lucindo C. de Pina, Amanda M. Rêgo, Nicole V. Ferreira, Paulo Redner, Luis Caetano M. Antunes

   Pages 101-135

6. Advanced Microbial Strain Subtyping Techniques for Molecular Epidemiology Investigations

   • Lee W. Riley

   Pages 137-163

7. Molecular Detection and Characterization of Carbapenem-Resistant Enterobacteriaceae

   • Siqiang Niu, Liang Chen

   Pages 165-185

8. Advanced Methods for Screening and Identification of Methicillin-Resistant Staphylococcus aureus

   • Raymond Widen, Yi-Wei Tang

   Pages 187-198

9. Advanced Techniques in Diagnostic Parasitology

   • Bobbi S. Pritt

   Pages 199-218

10. Advanced Methods for Detection of Foodborne Pathogens

    • Heather Harbottle

    Pages 219-239

11. Technical and Clinical Niches for Point-of-Care Molecular Devices

    • Glen Hansen, Ellen Jo Baron

    Pages 241-267

12. The Human Coronaviruses

    • Oliver Schildgen

    Pages 269-280

13. The Role of the Human Bocavirus (HBoV) in Respiratory Infections

    • Oliver Schildgen, Verena Schildgen

    Pages 281-301

14. Technical Advances in Veterinary Diagnostic Microbiology

    • Dongyou Liu

    Pages 303-316

15. Recent Advances in Veterinary Diagnostic Virology

    • Anbu K. Karuppannan, Alessandra Marnie M. G. de Castro, Tanja Opriessnig

    Pages 317-344

16. Detection of Viral RNA Splicing in Diagnostic Virology

    • Vladimir Majerciak, Zhi-Ming Zheng

    Pages 345-402

17. Advanced Molecular Diagnosis of Fungal Infections

    • Nathan P. Wiederhold

    Pages 403-421

18. Laboratory Technical Advances in the Diagnosis of Clostridium difficile

    • Masako Mizusawa, Karen C. Carroll

    Pages 423-443

19. Laboratory Diagnosis of HIV-1 Infections: State of the Art

    • Nang L. Nguyen, Rodney Arcenas, Yi-Wei Tang

    Pages 445-473

20. Multiplex PCR for Detection and Identification of Microbial Pathogens

    • Mark A. Poritz, Beth Lingenfelter

    Pages 475-493

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions. 

• microbial infection
• PCR
• diagnosis
• next generation sequencing
• clinical relevance

• Departments of Laboratory Medicine and Internal Medicine, Memorial Sloan Kettering Cancer Center, New York, USA

  Yi-Wei Tang

• Department of Pathology, Microbiology and Immunology and Medicine, Vanderbilt University Medical Center, Nashville, USA

  Charles W. Stratton

Yi-Wei Tang, Chief of Clinical Microbiology Service, Memorial Sloan-Kettering Cancer Center

Charles W. Stratton, Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine