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  • Textbook
  • © 2018

Cell Culture Technology


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  • Established in cooperations with postdoctoral scientists

  • Written by international experts

  • Focuses on modern, mammalian cell culture techniques and applications

  • Provides insights into the use of human cells including stem cells

  • Includes exercises to enable readers to test their understanding

Part of the book series: Learning Materials in Biosciences (LMB)

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USD 59.99
Price excludes VAT (USA)
  • ISBN: 978-3-319-74854-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 79.99
Price excludes VAT (USA)

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Table of contents (10 chapters)

  1. Front Matter

    Pages I-XII
  2. Cell Culture Bioprocess Technology: Biologics and Beyond

    • Sofie O’Brien, Yonsil Park, Samira Azarin, Wei-Shou Hu
    Pages 1-21
  3. Essentials in Cell Culture

    • Antonina Lavrentieva
    Pages 23-48
  4. Cell Culture Media

    • Reinhard Henschler
    Pages 49-59
  5. The Third Dimension in Cell Culture: From 2D to 3D Culture Formats

    • Verena Charwat, Dominik Egger
    Pages 75-90
  6. Biomaterials

    • Rainer Detsch, Julia Will, Jasmin Hum, Judith A. Roether, Aldo R. Boccaccini
    Pages 91-105
  7. Cell–Surface Interactions

    • Megan Livingston, F. Kurtis Kasper
    Pages 107-128
  8. Mimicking Physiological Oxygen in Cell Cultures

    • Nicholas R. Forsyth, Rachel Steeg, Muhammad Ahmad, Mohammed Al Zubaidi, Rakad Al-Jumaily, Marwan Merkhan et al.
    Pages 129-137
  9. Co-cultures

    • Roland Jacobs, Ralf Hass
    Pages 139-153
  10. Automation of Cell Culture Processes

    • Jan Hansmann, Ivo Schwedhelm, Andreas Clementi, Dominik Egger, Cornelia Kasper
    Pages 155-168

About this book

This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research.

The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells.

It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.


  • cell culture
  • 3 D, cell and tissue engineering
  • bioreactors
  • cell based testing
  • biochips
  • human cells

Editors and Affiliations

  • Department of Biotechnology, University of Natural Resources & Life Sciences, Vienna, Austria

    Cornelia Kasper, Verena Charwat

  • Institute for Technical Chemistry, Gottfried Wilhelm Leibniz Universität, Hannover, Germany

    Antonina Lavrentieva

About the editors

Cornelia Kasper studied Chemistry at the University Hannover and received her Diploma degree 1994. After PhD at the Institute for Technical Chemistry 1998 she was employed as EU liason officer at the University of Hannover. In 2007 she received Habilitation. 2000-2011 Cornelia Kasper has been head of Cell Culture and Tissue Engineering Group at the Institute for Technical Chemistry, Leibniz University of Hannover and head of young researcher group JRG “Large Scale Cultivation” within Cluster of Excellence “Rebirth” (from Regenerative Biology and Reconstructive Therapies). Since October 2011 Cornelia Kasper has been appointed as University Professor “Cell and Tissue Culture Technology” at the Department of Biotechnology at Boku. The focus in her research group is the isolation, characterization, expansion and differentiation of human primary/progenitor and stem cells. For the expansion and guided differentiation she develops optimized strategies and bioprocesses for 3 D cell culture conditions mimicking physiological environment generating functional cells for tissue engineering and cell based therapies and 3 D test systems under defined and controlled dynamic conditions.

Antonina Lavrentieva is a group leader at the Institute of Technical Chemistry, Leibniz University of Hannover, working in the field of stem cell research, biotesting and tissue engineering. In her second PhD Thesis, she studied methods of expanding mesenchymal stem cells (MSCs) in bioreactors, as well as the influence of hypoxia on the MSCs. She studied Biology and Life Science at Moscow State University and the University of Hannover. She also defended a PhD Thesis in Physiology. Her current research interests include stem cell media optimization, 3D cell culture, gradient hydrogels for studying cell niches and 3D-bioprinting.

Verena Charwat completed her bachelor studies in Medical Computer Science at the Vienna University of Technology in 2008 and her diploma studies in Bioengineering with specialization on biotechnological quality management at FH-Campus Wien in 2009. She continued her academic training with the Biomedical Ph.D. program at Medical University Vienna. Her Ph.D. thesis is entitled “Biosensors for label-free continuous monitoring of microfluidic cell cultures”. Since her graduation in 2014, she is working as a postdoctoral university assistant at the University of Natural Resources and Life Sciences, Vienna. Verena Charwat’s main research interest is in the field of biomedical engineering, with a special focus on the development and application of lab-on-a-chip systems with integrated biosensors for in vitro establishment and non-invasive monitoring of functional tissue mimics. Her research is targeted towards creation of physiologically relevant cell culture models including 3D cultivation, co-cultures and dynamic cultivation for basic biological research and biomedical applications.

Bibliographic Information

Buying options

USD 59.99
Price excludes VAT (USA)
  • ISBN: 978-3-319-74854-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 79.99
Price excludes VAT (USA)