Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

  • Marco Rito-Palomares
  • Jorge Benavides

Part of the Food Engineering Series book series (FSES)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Karla Mayolo-Deloisa, Jorge Benavides, Marco Rito-Palomares
    Pages 1-18
  3. José González-Valdez, Jorge Benavides, Marco Rito-Palomares
    Pages 19-33
  4. José González-Valdez, Karla Mayolo-Deloisa
    Pages 35-53
  5. Mirna González-González, Federico Ruiz-Ruiz
    Pages 55-78
  6. Juan Carlos Sánchez-Rangel, Daniel A. Jacobo-Velázquez, Jorge Benavides
    Pages 79-96
  7. Federico Ruiz-Ruiz, Jorge Benavides, Marco Rito-Palomares
    Pages 97-121
  8. Patricia Vázquez-Villegas, Oscar Aguilar
    Pages 141-159
  9. Mario A. Torres-Acosta, Federico Ruiz-Ruiz, Jorge Benavides, Marco Rito-Palomares
    Pages 161-178
  10. Jorge Benavides, Marco Rito-Palomares
    Pages 179-185
  11. Back Matter
    Pages 187-191

About this book


This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area.

Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market. 


Aqueous two-phase systems Primary recovery of biomolecules Downstream processing Bioseparation trends Low molecular weight compounds

Editors and affiliations

  • Marco Rito-Palomares
    • 1
  • Jorge Benavides
    • 2
  1. 1.Tecnologico de MonterreyEscuela de Ingeniería y Ciencias Centro de Biotecnología-FEMSAMonterreyMexico
  2. 2.Tecnologico de MonterreyEscuela de MedicinaMonterreyMexico

Bibliographic information